Restriction map for
pFN21AE0766
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstUI BstXI BstYI Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AlwI 292 GGATC GGATC(4/5)
ApaI 330 GGGCCC GGGCC^C
AspHI 359 GTGCTC G[AT]GC[AT]^C
AvaII 1262 GGACC G^G[AT]CC
BanII 330 GGGCCC G[GA]GC[CT]^C
BbsI 60 GTCTTC GAAGAC(2/6)
BpmI 39 CTCCAG CTGGAG(16/14)
BpuAI 60 GTCTTC GAAGAC(2/6)
BsgI 370 GTGCAG GTGCAG(16/14)
BsiHKAI 359 GTGCTC G[AT]GC[AT]^C
BspMI 452 ACCTGC ACCTGC(4/8)
BssKI 523 CCAGG ^CC[ACGT]GG
BstNI 523 CCAGG CC^[AT]GG
DraII 330 GGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 268 GACGTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 309 CCATGG C^C[GA][CT]GG
EarI 1307 GAAGAG CTCTTC(1/4)
EcoO109I 330 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 523 CCAGG ^CC[AT]GG
Ksp632I 1307 GAAGAG CTCTTC(1/4)
MspA1I 68 CAGCTG C[AC]G^C[GT]G
MvaI 523 CCAGG CC^[AT]GG
NcoI 309 CCATGG C^CATGG
NheI 76 GCTAGC G^CTAGC
NlaIV 330 GGGCCC GG[ACGT]^[ACGT]CC
PleI 21 GACTC GAGTC(4/5)
PvuII 68 CAGCTG CAG^CTG
ScrFI 523 CCAGG CC^[ACGT]GG
SexAI 522 ACCAGGT A^CC[AT]GGT
TaqI 222 TCGA T^CGA
TthHB8I 222 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 1225 CCGC CCGC(-3/-1)
429 GCGG
BfaI 77 CTAG C^TAG
146 CTAG
BsaJI 81 CCAAGG C^C[ACGT][ACGT]GG
309 CCATGG
BseRI 398 CTCCTC GAGGAG(10/8)
401 CTCCTC
BsiYI 81 CCAAGGCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1233 CCCTGTTGGGG
BslI 81 CCAAGGCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1233 CCCTGTTGGGG
BsmAI 19 GAGAC GTCTC(1/5)
1310 GAGAC
Cac8I 49 GCCAGC GC[ACGT]^[ACGT]GC
76 GCTAGC
DpnII 293 GATC ^GATC
662 GATC
EcoT14I 81 CCAAGG C^C[AT][AT]GG
309 CCATGG
HaeIII 48 GGCC GG^CC
331 GGCC
MaeI 77 CTAG C^TAG
146 CTAG
MboI 293 GATC ^GATC
662 GATC
NdeII 293 GATC ^GATC
662 GATC
PstI 71 CTGCAG CTGCA^G
454 CTGCAG
Sau3AI 293 GATC ^GATC
662 GATC
SfaNI 142 GATGC GCATC(5/9)
1218 GATGC
SfcI 71 CTGCAG C^T[GA][CT]AG
454 CTGCAG
StyI 81 CCAAGG C^C[AT][AT]GG
309 CCATGG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
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AccI AcsI AluI ApoI BbvI
BmyI Bsp1286I BsrI Bst1107I BstAPI
BstEII BstF5I BstPI BstZ17I Cfr13I
DdeI EcoO65I EcoRV Fnu4HI FokI
HinfI HphI ItaI MaeII MaeIII
MboII MnlI MseI MslI MwoI
NlaIII Sau96I TfiI Tru9I TseI
Tsp45I Tsp509I TspRI