Restriction map for
pFN21AE3517
##################################
# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII Alw44I
AlwI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BcnI BfrI
BglII BlnI BlpI BmyI Bpu1102I
BsaBI BsaHI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraI DraII DraIII
DsaI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MboI MfeI MflI
MluI MroI MslI MunI MvnI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StyI SwaI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflIII 378 ACACGT A^C[GA][CT]GT
AgeI 526 ACCGGT A^CCGGT
AhdI 196 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 79 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 269 AGCGCT AGC^GCT
AspEI 196 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 225 GGACC G^G[AT]CC
BalI 261 TGGCCA TGG^CCA
BbrPI 379 CACGTG CAC^GTG
BbsI 126 GAAGAC GAAGAC(2/6)
BfaI 290 CTAG C^TAG
BglI 172 GCCTGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 52 CTGGAG CTGGAG(16/14)
BpuAI 126 GAAGAC GAAGAC(2/6)
BsaAI 379 CACGTG [CT]AC^GT[GA]
BsaI 511 GAGACC GGTCTC(1/5)
BsaJI 108 CCTGGG C^C[ACGT][ACGT]GG
BsaWI 526 ACCGGT [AT]^CCGG[AT]
BseRI 637 GAGGAG GAGGAG(10/8)
Bsp1407I 152 TGTACA T^GTACA
BsrBI 120 GAGCGG CCGCTC(-3/-3)
BsrFI 526 ACCGGT [GA]^CCGG[CT]
BsrGI 152 TGTACA T^GTACA
BstXI 212 CCATGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 526 ACCGGT [GA]^CCGG[CT]
Cfr13I 225 GGACC G^G[ACGT]CC
DdeI 206 CTAAG C^T[ACGT]AG
DrdI 400 GACGATGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 261 TGGCCA [CT]^GGCC[GA]
Eam1105I 196 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 269 AGCGCT AGC^GCT
FauI 122 GCGGG CCCGC(4/6)
HaeII 269 AGCGCT [GA]GCGC^[CT]
MaeI 290 CTAG C^TAG
MluNI 261 TGGCCA TGG^CCA
MscI 261 TGGCCA TGG^CCA
MseI 353 TTAA T^TAA
PinAI 526 ACCGGT A^CCGGT
PleI 640 GAGTC GAGTC(4/5)
PmaCI 379 CACGTG CAC^GTG
PmlI 379 CACGTG CAC^GTG
Sau96I 225 GGACC G^G[ACGT]CC
SspBI 152 TGTACA T^GTACA
StuI 23 AGGCCT AGG^CCT
Tru9I 353 TTAA T^TAA
XcmI 177 CCAGGCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 144 CCGC CCGC(-3/-1)
122 GCGG
AfaI 153 GTAC GT^AC
216 GTAC
BsiYI 552 CCGGCGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
598 CCAAAAGAGGG
BslI 552 CCGGCGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
598 CCAAAAGAGGG
BsmFI 251 GGGAC GGGAC(10/14)
607 GGGAC
BstF5I 131 CATCC GGATG(2/0)
29 GGATG
CfoI 170 GCGC GCG^C
270 GCGC
FokI 131 CATCC GGATG(9/13)
29 GGATG
HhaI 170 GCGC GCG^C
270 GCGC
HinP1I 170 GCGC G^CGC
270 GCGC
MaeII 380 ACGT A^CGT
536 ACGT
MboII 126 GAAGA GAAGA(8/7)
475 GAAGA
MspA1I 238 CAGCTG C[AC]G^C[GT]G
257 CAGCTG
PstI 241 CTGCAG CTGCA^G
461 CTGCAG
PvuII 238 CAGCTG CAG^CTG
257 CAGCTG
RsaI 153 GTAC GT^AC
216 GTAC
SfcI 241 CTGCAG C^T[GA][CT]AG
461 CTGCAG
TaqI 620 TCGA T^CGA
635 TCGA
TfiI 304 GATTC G^A[AT]TC
628 GAATC
TthHB8I 620 TCGA T^CGA
635 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI BbvI BsmAI BsrI BssKI
BstNI Cac8I EcoRII Fnu4HI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MnlI MspI MvaI MwoI
NlaIII ScrFI TseI Tsp45I Tsp509I
TspRI