##################################
# NO CUT ENZYMES (  231 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        AluI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        Fnu4HI      
FokI        FseI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
HphI        ItaI        KasI        KpnI        KspI        
MaeI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MspI        MunI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIII      NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        Sau3AI      Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        StyI        SwaI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   21 enzymes) #
###################################

AspI           97  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII          42  GAGCCC               G[GA]GC[CT]^C
BbsI          171  GAAGAC               GAAGAC(2/6)
BmyI           42  GAGCCC               G[AGT]GC[ACT]^C
BpmI          199  CTGGAG               CTGGAG(16/14)
BpuAI         171  GAAGAC               GAAGAC(2/6)
BsmAI          82  GAGAC                GTCTC(1/5)
BsmFI          95  GGGAC                GGGAC(10/14)
Bsp1286I       42  GAGCCC               G[AGT]GC[ACT]^C
HinfI          52  GACTC                G^A[ACGT]TC
MaeIII         50  GTGAC                ^GT[ACGT]AC
PflFI          97  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI           52  GACTC                GAGTC(4/5)
SapI           39  GAAGAGC              GCTCTTC(1/4)
SfaNI          75  GATGC                GCATC(5/9)
SfcI          165  CTACAG               C^T[GA][CT]AG
SspI          140  AATATT               AAT^ATT
TaqI          107  TCGA                 T^CGA
Tsp45I         50  GTGAC                ^GT[GC]AC
Tth111I        97  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       107  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

BsrI          198  ACTGG                ACTGG(1/-1)
              210  CCAGT               
DdeI           99  CTTAG                C^T[ACGT]AG
              176  CTAAG               
EarI           33  GAAGAG               CTCTTC(1/4)
               39  GAAGAG              
Ksp632I        33  GAAGAG               CTCTTC(1/4)
               39  GAAGAG              
MnlI          105  CCTC                 CCTC(7/6)
               36  GAGG                


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

MboII