Restriction map for
pFN21AE5016
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI AocI Aor51HI ApoI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpuAI BsaBI BsaWI BseAI
BseRI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI HincII HindII
HindIII HinfI HpaI KpnI Ksp632I
MaeIII MamI MboII MfeI MflI
MluI MroI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SalI SapI ScaI SexAI
SfaNI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI TaqI TfiI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I Van91I XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Alw44I 138 GTGCAC G^TGCAC
AlwI 937 GATCC GGATC(4/5)
ApaLI 138 GTGCAC G^TGCAC
AscI 465 GGCGCGCC GG^CGCGCC
AviII 434 TGCGCA TGC^GCA
BalI 161 TGGCCA TGG^CCA
BbeI 115 GGCGCC GGCGC^C
BbrPI 141 CACGTG CAC^GTG
BlpI 714 GCTCAGC GC^T[ACGT]AGC
BpmI 472 CTGGAG CTGGAG(16/14)
Bpu1102I 714 GCTCAGC GC^T[ACGT]AGC
BsaAI 141 CACGTG [CT]AC^GT[GA]
BsaI 915 GAGACC GGTCTC(1/5)
BsiEI 28 CGGCCG CG[GA][CT]^CG
BsmBI 679 GAGACG CGTCTC(1/5)
BsrDI 754 CATTGC GCAATG(2/0)
BsrI 399 ACTGG ACTGG(1/-1)
BstXI 383 CCAACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 714 GCTCAGC GC^T[ACGT]AGC
DpnII 937 GATC ^GATC
DraIII 322 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 28 CGGCCG C^GGCCG
EclXI 28 CGGCCG C^GGCCG
Eco52I 28 CGGCCG C^GGCCG
FseI 806 GGCCGGCC GGCCGG^CC
FspI 434 TGCGCA TGC^GCA
HphI 860 TCACC GGTGA(8/7)
KasI 115 GGCGCC G^GCGCC
MaeII 142 ACGT A^CGT
MboI 937 GATC ^GATC
MluNI 161 TGGCCA TGG^CCA
MscI 161 TGGCCA TGG^CCA
NarI 115 GGCGCC GG^CGCC
NdeII 937 GATC ^GATC
NspI 330 GCATGT [GA]CATG^[CT]
PmaCI 141 CACGTG CAC^GTG
PmlI 141 CACGTG CAC^GTG
Sau3AI 937 GATC ^GATC
SbfI 519 CCTGCAGG CCTGCA^GG
SfoI 115 GGCGCC GGC^GCC
Sse8387I 519 CCTGCAGG CCTGCA^GG
StuI 156 AGGCCT AGG^CCT
TspRI 398 CACTG CA[GC]TG[ACGT][ACGT]^
XbaI 576 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwNI 292 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
364 CAGCTGCTG
ApaI 6 GGGCCC GGGCC^C
74 GGGCCC
AspHI 138 GTGCAC G[AT]GC[AT]^C
685 GTGCTC
BfaI 487 CTAG C^TAG
577 CTAG
BsgI 319 CTGCAC GTGCAG(16/14)
586 CTGCAC
BsiHKAI 138 GTGCAC G[AT]GC[AT]^C
685 GTGCTC
BsmAI 679 GAGAC GTCTC(1/5)
915 GAGAC
BspMI 302 ACCTGC ACCTGC(4/8)
590 ACCTGC
BstAPI 321 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
654 GCACCCGCTGC
EcoT14I 61 CCTTGG C^C[AT][AT]GG
604 CCTTGG
KspI 479 CCGCGG CCGC^GG
965 CCGCGG
MaeI 487 CTAG C^TAG
577 CTAG
MslI 141 CACGTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
949 CACGGCTGTG
NlaIII 79 CATG CATG^
331 CATG
PpuMI 106 GGGTCCC [GA]G^G[AT]CC[CT]
779 GGGACCT
PstI 361 CTGCAG CTGCA^G
520 CTGCAG
SacII 479 CCGCGG CCGC^GG
965 CCGCGG
SmaI 198 CCCGGG CCC^GGG
972 CCCGGG
StyI 61 CCTTGG C^C[AT][AT]GG
604 CCTTGG
XmaCI 198 CCCGGG C^CCGGG
972 CCCGGG
XmaI 198 CCCGGG C^CCGGG
972 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BsaHI BsaJI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BssHII BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NgoMIV NlaIV
PvuII Sau96I ScrFI SfcI TseI