Restriction map for
pFN21AE1717
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AvaI
AviII AvrII BanI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FokI FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MaeIII MfeI MluI
MroI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tru9I Tsp45I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 268 GTCGAC GT^[AC][GT]AC
AlwNI 430 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 562 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 102 TGGCCA TGG^CCA
BamHI 398 GGATCC G^GATCC
BanII 352 GGGCTC G[GA]GC[CT]^C
BbsI 19 GAAGAC GAAGAC(2/6)
BbvCI 594 CCTCAGC CCTCAGC(-5/-2)
BglI 473 GCCCCAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 638 AGATCT A^GATCT
BpuAI 19 GAAGAC GAAGAC(2/6)
BsaBI 393 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 587 GTGCAG GTGCAG(16/14)
BsmFI 554 GGGAC GGGAC(10/14)
BstAPI 542 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 453 GCGC GCG^C
DraII 554 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 177 GACAGGAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 74 CCACGG C^C[GA][CT]GG
EcoO109I 554 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 41 CCAAGG C^C[AT][AT]GG
HaeII 452 GGCGCT [GA]GCGC^[CT]
HhaI 453 GCGC GCG^C
HinP1I 453 GCGC G^CGC
HincII 268 GTCGAC GT[CT]^[GA]AC
HindII 268 GTCGAC GT[CT]^[GA]AC
MamI 393 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 19 GAAGA GAAGA(8/7)
MluNI 102 TGGCCA TGG^CCA
MscI 102 TGGCCA TGG^CCA
MspA1I 244 CCGCTG C[AC]G^C[GT]G
NspI 86 GCATGC [GA]CATG^[CT]
PflFI 562 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 379 GACTC GAGTC(4/5)
PpuMI 554 GGGACCT [GA]G^G[AT]CC[CT]
SalI 268 GTCGAC G^TCGAC
SphI 86 GCATGC GCATG^C
StuI 591 AGGCCT AGG^CCT
StyI 41 CCAAGG C^C[AT][AT]GG
TfiI 522 GATTC G^A[AT]TC
Tsp509I 32 AATT ^AATT
Tth111I 562 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 299 CCAAGACCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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Asp700I 27 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
336 GAACTACTTC
AvaII 555 GGACC G^G[AT]CC
567 GGTCC
BmyI 191 GTGCCC G[AGT]GC[ACT]^C
352 GGGCTC
BpmI 111 CTCCAG CTGGAG(16/14)
355 CTCCAG
BseRI 316 GAGGAG GAGGAG(10/8)
610 GAGGAG
Bsp1286I 191 GTGCCC G[AGT]GC[ACT]^C
352 GGGCTC
BsrI 56 ACTGG ACTGG(1/-1)
402 CCAGT
BstYI 398 GGATCC [GA]^GATC[CT]
638 AGATCT
DdeI 595 CTCAG C^T[ACGT]AG
664 CTGAG
EcoNI 309 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
603 CCTGGCCGAGG
HinfI 379 GACTC G^A[ACGT]TC
522 GATTC
MflI 398 GGATCC [GA]^GATC[CT]
638 AGATCT
PstI 145 CTGCAG CTGCA^G
582 CTGCAG
SfaNI 116 GCATC GCATC(5/9)
482 GCATC
TaqI 119 TCGA T^CGA
269 TCGA
TspRI 403 CAGTG CA[GC]TG[ACGT][ACGT]^
585 CAGTG
TthHB8I 119 TCGA T^CGA
269 TCGA
XhoII 398 GGATCC [GA]^GATC[CT]
638 AGATCT
XmnI 27 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
336 GAACTACTTC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AlwI BbvI BcnI
BsaJI BsiYI BslI BssKI BstNI
Cac8I Cfr13I DpnII EaeI EcoRII
Fnu4HI HaeIII HapII HpaII HphI
ItaI MaeII MboI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfcI
TseI