Restriction map for
pFN21AE0892
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# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsoBI Bsp1286I BspDI BspEI BspHI
BsrBI BsrDI BsrFI BsrI BssHII
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FbaI FokI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MfeI MflI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AfaI 506 GTAC GT^AC
AflIII 474 ACATGT A^C[GA][CT]GT
AlwI 154 GATCC GGATC(4/5)
Asp700I 31 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 64 GAAGAC GAAGAC(2/6)
BbvCI 686 CCTCAGC CCTCAGC(-5/-2)
BfaI 433 CTAG C^TAG
BpuAI 64 GAAGAC GAAGAC(2/6)
BsaBI 331 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 924 GTCTC GTCTC(1/5)
BsmI 436 GCATTC GAATGC(1/-1)
Bsp1407I 505 TGTACA T^GTACA
BspLU11I 474 ACATGT A^CATGT
BspMI 718 ACCTGC ACCTGC(4/8)
BsrGI 505 TGTACA T^GTACA
BssSI 244 CTCGTG CACGAG(-5/-1)
Cac8I 609 GCTGGC GC[ACGT]^[ACGT]GC
DpnII 154 GATC ^GATC
EcoRI 659 GAATTC G^AATTC
MaeI 433 CTAG C^TAG
MamI 331 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 154 GATC ^GATC
MwoI 346 GCGGCTATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 154 GATC ^GATC
NspI 474 ACATGT [GA]CATG^[CT]
PstI 720 CTGCAG CTGCA^G
PvuII 692 CAGCTG CAG^CTG
RsaI 506 GTAC GT^AC
Sau3AI 154 GATC ^GATC
SexAI 104 ACCTGGT A^CC[AT]GGT
SmlI 676 CTCAAG C^T[CT][GA]AG
SspBI 505 TGTACA T^GTACA
TaqI 159 TCGA T^CGA
TthHB8I 159 TCGA T^CGA
XmnI 31 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 31 GAATTT [GA]^AATT[CT]
659 GAATTC
AlwNI 414 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
689 CAGCAGCTG
ApoI 31 GAATTT [GA]^AATT[CT]
659 GAATTC
AvaII 807 GGACC G^G[AT]CC
967 GGACC
BalI 353 TGGCCA TGG^CCA
593 TGGCCA
BseRI 51 CTCCTC GAGGAG(10/8)
567 CTCCTC
BsmFI 45 GGGAC GGGAC(10/14)
232 GTCCC
Cfr13I 807 GGACC G^G[ACGT]CC
967 GGACC
EaeI 353 TGGCCA [CT]^GGCC[GA]
593 TGGCCA
HincII 541 GTCAAC GT[CT]^[GA]AC
837 GTTGAC
HindII 541 GTCAAC GT[CT]^[GA]AC
837 GTTGAC
MboII 64 GAAGA GAAGA(8/7)
308 TCTTC
MluNI 353 TGGCCA TGG^CCA
593 TGGCCA
MscI 353 TGGCCA TGG^CCA
593 TGGCCA
MseI 871 TTAA T^TAA
920 TTAA
MspA1I 363 CCGCTG C[AC]G^C[GT]G
692 CAGCTG
NlaIV 624 GGCTCC GG[ACGT]^[ACGT]CC
967 GGACCC
PleI 23 GACTC GAGTC(4/5)
47 GACTC
Sau96I 807 GGACC G^G[ACGT]CC
967 GGACC
SfcI 720 CTGCAG C^T[GA][CT]AG
864 CTACAG
Tru9I 871 TTAA T^TAA
920 TTAA
Tsp45I 109 GTCAC ^GT[GC]AC
397 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AluI BbvI BssKI BstNI
DdeI EcoRII Fnu4HI HaeIII HinfI
HphI ItaI MaeIII MnlI MslI
MvaI NlaIII ScrFI SfaNI TfiI
TseI Tsp509I TspRI