Restriction map for
pFN21AE3338
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrFI BsrGI BssHII Bst1107I BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SacII SalI SapI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AclI 326 AACGTT AA^CGTT
BsmBI 1024 CGTCTC CGTCTC(1/5)
BsmI 984 GAATGC GAATGC(1/-1)
BspLU11I 2624 ACATGT A^CATGT
BspMI 818 ACCTGC ACCTGC(4/8)
BsrBI 923 GAGCGG CCGCTC(-3/-3)
BsrDI 2509 GCAATG GCAATG(2/0)
BssSI 785 CACGAG CACGAG(-5/-1)
BstAPI 2250 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 842 TTCGAA TT^CGAA
DsaI 2156 CCATGG C^C[GA][CT]GG
EcoNI 672 CCTCGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 897 GAATTC G^AATTC
EcoT14I 2156 CCATGG C^C[AT][AT]GG
NcoI 2156 CCATGG C^CATGG
NheI 2151 GCTAGC G^CTAGC
NspV 842 TTCGAA TT^CGAA
Psp1406I 326 AACGTT AA^CGTT
SacI 598 GAGCTC GAGCT^C
ScaI 1573 AGTACT AGT^ACT
SfuI 842 TTCGAA TT^CGAA
SphI 1148 GCATGC GCATG^C
StuI 2210 AGGCCT AGG^CCT
StyI 2156 CCATGG C^C[AT][AT]GG
TaqI 843 TCGA T^CGA
TthHB8I 843 TCGA T^CGA
XbaI 2659 TCTAGA T^CTAGA
XcmI 497 CCAGTGAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 528 ACGTGT A^C[GA][CT]GT
2624 ACATGT
Asp700I 663 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1309 GAAGTAATTC
AspHI 220 GAGCAC G[AT]GC[AT]^C
598 GAGCTC
BalI 485 TGGCCA TGG^CCA
2159 TGGCCA
BanII 598 GAGCTC G[GA]GC[CT]^C
2642 GAGCCC
BcnI 608 CCCGG CC^[GC]GG
2112 CCGGG
BglII 708 AGATCT A^GATCT
2025 AGATCT
BsaBI 409 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2902 GATTTTCATC
BsaI 353 GAGACC GGTCTC(1/5)
2680 GAGACC
BsgI 652 GTGCAG GTGCAG(16/14)
1106 GTGCAG
BsiHKAI 220 GAGCAC G[AT]GC[AT]^C
598 GAGCTC
CfoI 1428 GCGC GCG^C
2862 GCGC
EaeI 485 TGGCCA [CT]^GGCC[GA]
2159 TGGCCA
HapII 609 CCGG C^CGG
2112 CCGG
HhaI 1428 GCGC GCG^C
2862 GCGC
HinP1I 1428 GCGC G^CGC
2862 GCGC
HincII 277 GTCAAC GT[CT]^[GA]AC
2364 GTTGAC
HindII 277 GTCAAC GT[CT]^[GA]AC
2364 GTTGAC
HpaII 609 CCGG C^CGG
2112 CCGG
HphI 894 GGTGA GGTGA(8/7)
1461 TCACC
MaeII 327 ACGT A^CGT
528 ACGT
MamI 409 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2902 GATTTTCATC
MfeI 1882 CAATTG C^AATTG
2254 CAATTG
MluNI 485 TGGCCA TGG^CCA
2159 TGGCCA
MscI 485 TGGCCA TGG^CCA
2159 TGGCCA
MspI 609 CCGG C^CGG
2112 CCGG
MunI 1882 CAATTG C^AATTG
2254 CAATTG
NciI 608 CCCGG CC^[GC]GG
2112 CCGGG
SexAI 1253 ACCAGGT A^CC[AT]GGT
2357 ACCAGGT
XmnI 663 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1309 GAAGTAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanI BbsI
BbvCI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HinfI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII