Restriction map for
pFN21AE6260
##################################
# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI AlwI AlwNI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DpnII DraI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MboI MfeI
MflI MluI MroI MseI MslI
MunI MvnI NarI NcoI NdeI
NdeII NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tru9I Tsp509I TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Alw44I 327 GTGCAC G^TGCAC
Aor51HI 224 AGCGCT AGC^GCT
ApaLI 327 GTGCAC G^TGCAC
AspHI 327 GTGCAC G[AT]GC[AT]^C
BalI 255 TGGCCA TGG^CCA
BbrPI 321 CACGTG CAC^GTG
BglI 467 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 327 GTGCAC G[AGT]GC[ACT]^C
BsaAI 321 CACGTG [CT]AC^GT[GA]
BsgI 264 CTGCAC GTGCAG(16/14)
BsiHKAI 327 GTGCAC G[AT]GC[AT]^C
Bsp1286I 327 GTGCAC G[AGT]GC[ACT]^C
BsrFI 520 GCCGGC [GA]^CCGG[CT]
BsrI 393 CCAGT ACTGG(1/-1)
BstEII 375 GGTGACC G^GT[ACGT]ACC
BstF5I 129 GGATG GGATG(2/0)
BstPI 375 GGTGACC G^GT[ACGT]ACC
BstXI 353 CCAAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 520 GCCGGC [GA]^CCGG[CT]
DraIII 321 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 224 AGCGCT AGC^GCT
EcoO65I 375 GGTGACC G^GT[ACGT]ACC
EcoT14I 525 CCTTGG C^C[AT][AT]GG
FokI 129 GGATG GGATG(9/13)
HgaI 237 GACGC GACGC(5/10)
MaeII 322 ACGT A^CGT
MaeIII 376 GTGAC ^GT[ACGT]AC
MluNI 255 TGGCCA TGG^CCA
MscI 255 TGGCCA TGG^CCA
MspA1I 336 CAGCTG C[AC]G^C[GT]G
NaeI 520 GCCGGC GCC^GGC
NgoMIV 520 GCCGGC G^CCGGC
PmaCI 321 CACGTG CAC^GTG
PmlI 321 CACGTG CAC^GTG
PvuII 336 CAGCTG CAG^CTG
SmaI 470 CCCGGG CCC^GGG
StuI 300 AGGCCT AGG^CCT
StyI 525 CCTTGG C^C[AT][AT]GG
Tsp45I 376 GTGAC ^GT[GC]AC
XmaCI 470 CCCGGG C^CCGGG
XmaI 470 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BcnI 470 CCCGG CC^[GC]GG
471 CCGGG
BsaI 148 GAGACC GGTCTC(1/5)
546 GAGACC
BseRI 142 GAGGAG GAGGAG(10/8)
145 GAGGAG
BsmAI 148 GAGAC GTCTC(1/5)
546 GAGAC
DraII 367 GGGACCC [GA]G^G[ACGT]CC[CT]
440 AGGACCC
DsaI 96 CCACGG C^C[GA][CT]GG
282 CCACGG
EaeI 46 CGGCCA [CT]^GGCC[GA]
255 TGGCCA
EcoO109I 367 GGGACCC [GA]G^G[ACGT]CC[CT]
440 AGGACCC
HaeII 6 GGCGCT [GA]GCGC^[CT]
224 AGCGCT
HapII 471 CCGG C^CGG
521 CCGG
HinfI 295 GAGTC G^A[ACGT]TC
434 GAGTC
HpaII 471 CCGG C^CGG
521 CCGG
HphI 375 GGTGA GGTGA(8/7)
350 TCACC
MspI 471 CCGG C^CGG
521 CCGG
NciI 470 CCCGG CC^[GC]GG
471 CCGGG
NlaIII 253 CATG CATG^
381 CATG
PleI 295 GAGTC GAGTC(4/5)
434 GAGTC
PpuMI 367 GGGACCC [GA]G^G[AT]CC[CT]
440 AGGACCC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI AvaI AvaII BbvI
BsaJI BsiYI BslI BsmFI BsoBI
BspMI BsrDI BssKI BstNI Cac8I
CfoI Cfr13I EarI EcoRII Fnu4HI
HaeIII HhaI HinP1I ItaI Ksp632I
MboII MnlI MvaI MwoI NlaIV
Sau96I ScrFI TseI