Restriction map for
pFN21AE6474
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HpaII KasI
KpnI KspI MamI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 1267 GTCTAC GT^[AC][GT]AC
AfaI 351 GTAC GT^AC
AlwNI 815 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 571 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 233 GAGCTC G[AT]GC[AT]^C
BamHI 1039 GGATCC G^GATCC
BanII 233 GAGCTC G[GA]GC[CT]^C
BfaI 485 CTAG C^TAG
BpmI 530 CTGGAG CTGGAG(16/14)
BsaAI 1270 TACGTG [CT]AC^GT[GA]
BsaI 933 GAGACC GGTCTC(1/5)
BsgI 83 GTGCAG GTGCAG(16/14)
BsiHKAI 233 GAGCTC G[AT]GC[AT]^C
BsmI 582 GAATGC GAATGC(1/-1)
BssSI 449 CTCGTG CACGAG(-5/-1)
BstYI 1039 GGATCC [GA]^GATC[CT]
Cfr13I 960 GGGCC G^G[ACGT]CC
DsaI 269 CCACGG C^C[GA][CT]GG
EcoRI 437 GAATTC G^AATTC
EcoRV 607 GATATC GAT^ATC
EcoT14I 636 CCAAGG C^C[AT][AT]GG
EcoT22I 720 ATGCAT ATGCA^T
FauI 659 GCGGG CCCGC(4/6)
HincII 766 GTTAAC GT[CT]^[GA]AC
HindII 766 GTTAAC GT[CT]^[GA]AC
HindIII 216 AAGCTT A^AGCTT
HpaI 766 GTTAAC GTT^AAC
HphI 615 TCACC GGTGA(8/7)
MaeI 485 CTAG C^TAG
MflI 1039 GGATCC [GA]^GATC[CT]
NheI 484 GCTAGC G^CTAGC
NsiI 720 ATGCAT ATGCA^T
NspI 664 GCATGC [GA]CATG^[CT]
PleI 140 GAGTC GAGTC(4/5)
PstI 200 CTGCAG CTGCA^G
RsaI 351 GTAC GT^AC
SacI 233 GAGCTC GAGCT^C
Sau96I 960 GGGCC G^G[ACGT]CC
SphI 664 GCATGC GCATG^C
SspI 1381 AATATT AAT^ATT
StyI 636 CCAAGG C^C[AT][AT]GG
SwaI 921 ATTTAAAT ATTT^AAAT
TfiI 285 GAATC G^A[AT]TC
XcmI 104 CCAAAGGTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1039 GGATCC [GA]^GATC[CT]
XmnI 571 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwI 1040 GATCC GGATC(4/5)
1039 GGATC
AseI 70 ATTAAT AT^TAAT
1119 ATTAAT
AsnI 70 ATTAAT AT^TAAT
1119 ATTAAT
BglI 169 GCCATACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
953 GCCAAGAGGGC
BmyI 51 GGGCAC G[AGT]GC[ACT]^C
233 GAGCTC
BsiYI 223 CCATGCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
419 CCATGTTGTGG
BslI 223 CCATGCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
419 CCATGTTGTGG
BsmFI 99 GTCCC GGGAC(10/14)
385 GTCCC
Bsp1286I 51 GGGCAC G[AGT]GC[ACT]^C
233 GAGCTC
BssKI 797 CCAGG ^CC[ACGT]GG
1149 CCAGG
BstF5I 296 CATCC GGATG(2/0)
357 CATCC
BstNI 797 CCAGG CC^[AT]GG
1149 CCAGG
DpnII 841 GATC ^GATC
1040 GATC
DraI 922 TTTAAA TTT^AAA
1324 TTTAAA
EarI 810 CTCTTC CTCTTC(1/4)
999 CTCTTC
EcoRII 797 CCAGG ^CC[AT]GG
1149 CCAGG
FokI 296 CATCC GGATG(9/13)
357 CATCC
HinfI 140 GAGTC G^A[ACGT]TC
285 GAATC
Ksp632I 810 CTCTTC CTCTTC(1/4)
999 CTCTTC
MboI 841 GATC ^GATC
1040 GATC
MvaI 797 CCAGG CC^[AT]GG
1149 CCAGG
NdeII 841 GATC ^GATC
1040 GATC
PflMI 223 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
419 CCATGTTGTGG
PshBI 70 ATTAAT AT^TAAT
1119 ATTAAT
Sau3AI 841 GATC ^GATC
1040 GATC
ScrFI 797 CCAGG CC^[ACGT]GG
1149 CCAGG
SmlI 25 CTCAAG C^T[CT][GA]AG
250 CTCAAG
TspRI 228 CAGTG CA[GC]TG[ACGT][ACGT]^
491 CAGTG
Van91I 223 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
419 CCATGTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AcsI AluI ApoI BalI
BbvI BsaJI BsmAI BsrI Cac8I
DdeI EaeI Fnu4HI HaeIII ItaI
MaeII MaeIII MboII MluNI MnlI
MscI MseI MslI MspA1I MwoI
NlaIII NlaIV PvuII SfaNI SfcI
TaqI Tru9I TseI Tsp45I Tsp509I
TthHB8I