##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       Cfr10I      CpoI        DdeI        
DpnII       DraI        DraII       DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HapII       HgaI        Hin1I       
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MaeI        MaeII       MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       Sau3AI      SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TfiI        TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI            9  GTCGAC               GT^[AC][GT]AC
AfaI          223  GTAC                 GT^AC
AvaII         126  GGTCC                G^G[AT]CC
BbsI           73  GTCTTC               GAAGAC(2/6)
BbvI          232  GCAGC                GCAGC(8/12)
BpmI          103  CTGGAG               CTGGAG(16/14)
BpuAI          73  GTCTTC               GAAGAC(2/6)
BsaJI          54  CCGTGG               C^C[ACGT][ACGT]GG
BsmAI          19  GTCTC                GTCTC(1/5)
BspDI         155  ATCGAT               AT^CGAT
BsrI          225  ACTGG                ACTGG(1/-1)
BssKI         173  CCAGG                ^CC[ACGT]GG
BstNI         173  CCAGG                CC^[AT]GG
CfoI            4  GCGC                 GCG^C
Cfr13I        126  GGTCC                G^G[ACGT]CC
ClaI          155  ATCGAT               AT^CGAT
DrdI          118  GACTTATTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           54  CCGTGG               C^C[GA][CT]GG
EaeI          227  TGGCCG               [CT]^GGCC[GA]
EcoRII        173  CCAGG                ^CC[AT]GG
HaeII           3  GGCGCT               [GA]GCGC^[CT]
HaeIII        228  GGCC                 GG^CC
HhaI            4  GCGC                 GCG^C
HinP1I          4  GCGC                 G^CGC
HincII          9  GTCGAC               GT[CT]^[GA]AC
HindII          9  GTCGAC               GT[CT]^[GA]AC
HphI          115  GGTGA                GGTGA(8/7)
MaeIII        116  GTGAC                ^GT[ACGT]AC
MseI          209  TTAA                 T^TAA
MvaI          173  CCAGG                CC^[AT]GG
NlaIII        220  CATG                 CATG^
NlaIV          48  GGCTCC               GG[ACGT]^[ACGT]CC
NspI          219  GCATGT               [GA]CATG^[CT]
PshAI          12  GACCATAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI          223  GTAC                 GT^AC
SalI            9  GTCGAC               G^TCGAC
SapI          252  GAAGAGC              GCTCTTC(1/4)
Sau96I        126  GGTCC                G^G[ACGT]CC
ScrFI         173  CCAGG                CC^[ACGT]GG
Tru9I         209  TTAA                 T^TAA
TseI          232  GCAGC                G^C[AT]GC
Tsp45I        116  GTGAC                ^GT[GC]AC
Tsp509I       243  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AciI          230  CCGC                 CCGC(-3/-1)
               42  GCGG                
EarI          166  GAAGAG               CTCTTC(1/4)
              252  GAAGAG              
Fnu4HI        229  GCCGC                GC^[ACGT]GC
              232  GCAGC               
ItaI          229  GCCGC                GC^[ACGT]GC
              232  GCAGC               
Ksp632I       166  GAAGAG               CTCTTC(1/4)
              252  GAAGAG              
MwoI           33  GCAGATAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               81  GCGAAAGAAGC         
TaqI           10  TCGA                 T^CGA
              156  TCGA                
TthHB8I        10  TCGA                 T^CGA
              156  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

HinfI       MboII       MnlI        PleI