##################################
# NO CUT ENZYMES (  200 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstNI       BstPI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DdeI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FseI        FspI        
HaeII       HapII       Hin1I       HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspI        MunI        MvaI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        ScrFI       SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tru9I       
Tsp509I     TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AlwI          206  GATCC                GGATC(4/5)
BanI          147  GGTGCC               G^G[CT][GA]CC
BbsI           88  GTCTTC               GAAGAC(2/6)
BclI           41  TGATCA               T^GATCA
BpuAI          88  GTCTTC               GAAGAC(2/6)
BsiYI         120  CCGCATTCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          120  CCGCATTCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         187  GGGAC                GGGAC(10/14)
BsmI          122  GCATTC               GAATGC(1/-1)
BssHII         72  GCGCGC               G^CGCGC
BstF5I         29  GGATG                GGATG(2/0)
BstYI         205  AGATCC               [GA]^GATC[CT]
Cac8I          72  GCGCGC               GC[ACGT]^[ACGT]GC
Cfr13I          3  GGGCC                G^G[ACGT]CC
EarI          193  GAAGAG               CTCTTC(1/4)
FbaI           41  TGATCA               T^GATCA
FokI           29  GGATG                GGATG(9/13)
HaeIII          4  GGCC                 GG^CC
HgaI           24  GACGC                GACGC(5/10)
HphI           58  TCACC                GGTGA(8/7)
Ksp632I       193  GAAGAG               CTCTTC(1/4)
MaeII          86  ACGT                 A^CGT
MaeIII         57  GTCAC                ^GT[ACGT]AC
MflI          205  AGATCC               [GA]^GATC[CT]
MnlI          213  GAGG                 CCTC(7/6)
MspA1I        224  CAGCTG               C[AC]G^C[GT]G
MwoI           27  GCGGATGCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV         147  GGTGCC               GG[ACGT]^[ACGT]CC
NspI           36  GCATGT               [GA]CATG^[CT]
PleI          130  GAGTC                GAGTC(4/5)
PvuII         224  CAGCTG               CAG^CTG
Sau96I          3  GGGCC                G^G[ACGT]CC
SfaNI          30  GATGC                GCATC(5/9)
SfcI           48  CTACAG               C^T[GA][CT]AG
Tsp45I         57  GTCAC                ^GT[GC]AC
XhoII         205  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BbvI           69  GCAGC                GCAGC(8/12)
              226  GCTGC               
DpnII          42  GATC                 ^GATC
              206  GATC                
MboI           42  GATC                 ^GATC
              206  GATC                
NdeII          42  GATC                 ^GATC
              206  GATC                
NlaIII         37  CATG                 CATG^
              182  CATG                
Sau3AI         42  GATC                 ^GATC
              206  GATC                
TaqI           65  TCGA                 T^CGA
              200  TCGA                
TseI           69  GCAGC                G^C[AT]GC
              226  GCTGC               
TthHB8I        65  TCGA                 T^CGA
              200  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   13 enzymes) #
#########################################

AccII       AciI        AluI        BstUI       CfoI        
Fnu4HI      HhaI        HinP1I      HinfI       ItaI        
MboII       MvnI        TfiI