##################################
# NO CUT ENZYMES (  220 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BcgI        BclI        BcnI        BfaI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssKI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoRV       
EcoT22I     FbaI        FokI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        HpaII       HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MspI        MunI        MvaI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
ScrFI       SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AlwI          124  GGATC                GGATC(4/5)
Asp700I       146  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII          91  GAGCCC               G[GA]GC[CT]^C
BbvCI          46  CCTCAGC              CCTCAGC(-5/-2)
BbvI          228  GCAGC                GCAGC(8/12)
BciVI         237  GGATAC               GTATCC(6/5)
BglI           51  GCCTAAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI           91  GAGCCC               G[AGT]GC[ACT]^C
BsaI          131  GAGACC               GGTCTC(1/5)
BsaJI         185  CCAAGG               C^C[ACGT][ACGT]GG
BsmAI         131  GAGAC                GTCTC(1/5)
BsmI          206  GAATGC               GAATGC(1/-1)
Bsp1286I       91  GAGCCC               G[AGT]GC[ACT]^C
BssSI         232  CTCGTG               CACGAG(-5/-1)
Cac8I         210  GCCTGC               GC[ACGT]^[ACGT]GC
DdeI           47  CTCAG                C^T[ACGT]AG
DpnII         125  GATC                 ^GATC
EcoT14I       185  CCAAGG               C^C[AT][AT]GG
FauI          247  GCGGG                CCCGC(4/6)
Fnu4HI        228  GCAGC                GC^[ACGT]GC
ItaI          228  GCAGC                GC^[ACGT]GC
MboI          125  GATC                 ^GATC
MwoI           51  GCCTAAAAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         125  GATC                 ^GATC
NlaIII        118  CATG                 CATG^
Sau3AI        125  GATC                 ^GATC
SfaNI         225  GATGC                GCATC(5/9)
StyI          185  CCAAGG               C^C[AT][AT]GG
TseI          228  GCAGC                G^C[AT]GC
TspRI         141  CAGTG                CA[GC]TG[ACGT][ACGT]^
XmnI          146  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

AciI           66  GCGG                 CCGC(-3/-1)
              247  GCGG                
AcsI            7  GAATTC               [GA]^AATT[CT]
              150  GAATTC              
ApoI            7  GAATTC               [GA]^AATT[CT]
              150  GAATTC              
EcoRI           7  GAATTC               G^AATTC
              150  GAATTC              
Tsp509I         8  AATT                 ^AATT
              151  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

AluI        MnlI