Restriction map for
pFN21AE0898
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# NO CUT ENZYMES ( 75 enzymes) #
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AatII AccI AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AspEI AspI AviII
AvrII BamHI BbeI BcgI BciVI
BfrI BlnI BsaHI BsaI BsiWI
BspDI BspLU11I BsrBI BssHII BssSI
Bst1107I BstBI BstXI BstZ17I Bsu36I
ClaI CpoI DraIII Eam1105I Eco47III
Eco81I EcoNI FspI HgaI Hin1I
KasI MluI MslI NarI NheI
NruI NspV PacI PflFI PflMI
PinAI PmeI PvuI RsrII SacI
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
Tth111I Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 70 enzymes) #
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Acc65I 1812 GGTACC G^GTACC
AccII 39 CGCG CG^CG
AccIII 1988 TCCGGA T^CCGGA
AclI 2171 AACGTT AA^CGTT
AflIII 3033 ACGTGT A^C[GA][CT]GT
AlwNI 1309 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1812 GGTACC G^GTACC
AspHI 2450 GAGCAC G[AT]GC[AT]^C
BalI 3383 TGGCCA TGG^CCA
BanII 169 GAGCCC G[GA]GC[CT]^C
BbrPI 1460 CACGTG CAC^GTG
BbvCI 3121 CCTCAGC CCTCAGC(-5/-2)
BglI 97 GCCGGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3363 AGATCT A^GATCT
BlpI 1306 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1306 GCTCAGC GC^T[ACGT]AGC
BsaWI 1988 TCCGGA [AT]^CCGG[AT]
BseAI 1988 TCCGGA T^CCGGA
BsgI 1601 CTGCAC GTGCAG(16/14)
BsiEI 35 CGGCCG CG[GA][CT]^CG
BsiHKAI 2450 GAGCAC G[AT]GC[AT]^C
BspEI 1988 TCCGGA T^CCGGA
BspMI 1780 GCAGGT ACCTGC(4/8)
BstAPI 3361 GCAGATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 457 GGTGACC G^GT[ACGT]ACC
BstPI 457 GGTGACC G^GT[ACGT]ACC
BstUI 39 CGCG CG^CG
CelII 1306 GCTCAGC GC^T[ACGT]AGC
CfoI 2931 GCGC GCG^C
DrdI 3315 GACAGAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 35 CGGCCG C^GGCCG
EclXI 35 CGGCCG C^GGCCG
Eco52I 35 CGGCCG C^GGCCG
EcoO65I 457 GGTGACC G^GT[ACGT]ACC
EcoRV 1979 GATATC GAT^ATC
EcoT22I 3419 ATGCAT ATGCA^T
FauI 2949 GCGGG CCCGC(4/6)
FseI 131 GGCCGGCC GGCCGG^CC
HaeII 2930 AGCGCC [GA]GCGC^[CT]
HhaI 2931 GCGC GCG^C
HinP1I 2931 GCGC G^CGC
HincII 3513 GTTAAC GT[CT]^[GA]AC
HindII 3513 GTTAAC GT[CT]^[GA]AC
HindIII 4027 AAGCTT A^AGCTT
HpaI 3513 GTTAAC GTT^AAC
KpnI 1812 GGTACC GGTAC^C
KspI 38 CCGCGG CCGC^GG
MluNI 3383 TGGCCA TGG^CCA
MroI 1988 TCCGGA T^CCGGA
MscI 3383 TGGCCA TGG^CCA
MvnI 39 CGCG CG^CG
NcoI 3386 CCATGG C^CATGG
NdeI 626 CATATG CA^TATG
NotI 34 GCGGCCGC GC^GGCCGC
NsiI 3419 ATGCAT ATGCA^T
PaeR7I 2000 CTCGAG C^TCGAG
PmaCI 1460 CACGTG CAC^GTG
PmlI 1460 CACGTG CAC^GTG
PpuMI 635 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 2901 GACGTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2171 AACGTT AA^CGTT
SacII 38 CCGCGG CCGC^GG
ScaI 1804 AGTACT AGT^ACT
SexAI 2017 ACCAGGT A^CC[AT]GGT
SmlI 2000 CTCGAG C^T[CT][GA]AG
StuI 559 AGGCCT AGG^CCT
SwaI 2462 ATTTAAAT ATTT^AAAT
Tsp45I 458 GTGAC ^GT[GC]AC
XcmI 2934 CCAGGGAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2000 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AseI 268 ATTAAT AT^TAAT
2386 ATTAAT
AsnI 268 ATTAAT AT^TAAT
2386 ATTAAT
AvaI 1558 CCCGAG C^[CT]CG[GA]G
2000 CTCGAG
BanI 1812 GGTACC G^G[CT][GA]CC
3265 GGTGCC
BclI 302 TGATCA T^GATCA
3134 TGATCA
BcnI 48 CCGGG CC^[GC]GG
98 CCGGG
BmyI 169 GAGCCC G[AGT]GC[ACT]^C
2450 GAGCAC
BsaAI 1460 CACGTG [CT]AC^GT[GA]
3032 TACGTG
BsaBI 2461 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3566 GATTTTCATC
BsoBI 1558 CCCGAG C^[CT]CG[GA]G
2000 CTCGAG
Bsp1286I 169 GAGCCC G[AGT]GC[ACT]^C
2450 GAGCAC
Bsp1407I 2032 TGTACA T^GTACA
2978 TGTACA
BspHI 1083 TCATGA T^CATGA
2112 TCATGA
BsrFI 132 GCCGGC [GA]^CCGG[CT]
157 GCCGGC
BsrGI 2032 TGTACA T^GTACA
2978 TGTACA
Cfr10I 132 GCCGGC [GA]^CCGG[CT]
157 GCCGGC
DraII 635 AGGACCC [GA]G^G[ACGT]CC[CT]
3959 AGGCCCC
DsaI 38 CCGCGG C^C[GA][CT]GG
3386 CCATGG
EcoO109I 635 AGGACCC [GA]G^G[ACGT]CC[CT]
3959 AGGCCCC
EcoRI 741 GAATTC G^AATTC
1965 GAATTC
FbaI 302 TGATCA T^GATCA
3134 TGATCA
MamI 2461 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3566 GATTTTCATC
MfeI 1739 CAATTG C^AATTG
3341 CAATTG
MunI 1739 CAATTG C^AATTG
3341 CAATTG
NaeI 132 GCCGGC GCC^GGC
157 GCCGGC
NciI 48 CCGGG CC^[GC]GG
98 CCGGG
NgoMIV 132 GCCGGC G^CCGGC
157 GCCGGC
PshBI 268 ATTAAT AT^TAAT
2386 ATTAAT
RcaI 1083 TCATGA T^CATGA
2112 TCATGA
SapI 538 GAAGAGC GCTCTTC(1/4)
2939 GAAGAGC
SphI 1298 GCATGC GCATG^C
2502 GCATGC
SspBI 2032 TGTACA T^GTACA
2978 TGTACA
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
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AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BbsI BbvI
BfaI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII
XmnI