##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwI        AlwNI       Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BcgI        BclI        BcnI        BfrI        BglII       
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmFI       BsoBI       
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrFI       BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstXI       BstYI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoRI       EcoRV       FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
TaqI        Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AflIII       1208  ACATGT               A^C[GA][CT]GT
AocI          379  CCTCAGG              CC^T[ACGT]AGG
AvrII         804  CCTAGG               C^CTAGG
BanI          135  GGTGCC               G^G[CT][GA]CC
BbvCI        1927  GCTGAGG              CCTCAGC(-5/-2)
BfaI          805  CTAG                 C^TAG
BglI          947  GCCACTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          804  CCTAGG               C^CTAGG
BseRI         479  GAGGAG               GAGGAG(10/8)
Bsp1407I      569  TGTACA               T^GTACA
BspLU11I     1208  ACATGT               A^CATGT
BsrGI         569  TGTACA               T^GTACA
BssKI         391  CCTGG                ^CC[ACGT]GG
BstAPI        316  GCATTTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1156  GGTGACC              G^GT[ACGT]ACC
BstNI         391  CCTGG                CC^[AT]GG
BstPI        1156  GGTGACC              G^GT[ACGT]ACC
Bsu36I        379  CCTCAGG              CC^T[ACGT]AGG
Cac8I         296  GCTCGC               GC[ACGT]^[ACGT]GC
DpnII         926  GATC                 ^GATC
EaeI          703  CGGCCA               [CT]^GGCC[GA]
EarI          368  CTCTTC               CTCTTC(1/4)
Eco81I        379  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      1156  GGTGACC              G^GT[ACGT]ACC
EcoRII        391  CCTGG                ^CC[AT]GG
Ksp632I       368  CTCTTC               CTCTTC(1/4)
MaeI          805  CTAG                 C^TAG
MaeII         904  ACGT                 A^CGT
MboI          926  GATC                 ^GATC
MvaI          391  CCTGG                CC^[AT]GG
NdeII         926  GATC                 ^GATC
NlaIV         135  GGTGCC               GG[ACGT]^[ACGT]CC
PflMI         646  CCATCCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau3AI        926  GATC                 ^GATC
ScrFI         391  CCTGG                CC^[ACGT]GG
SmlI         1372  CTTGAG               C^T[CT][GA]AG
SspBI         569  TGTACA               T^GTACA
SwaI          242  ATTTAAAT             ATTT^AAAT
Van91I        646  CCATCCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AspHI         294  GAGCTC               G[AT]GC[AT]^C
             1270  GAGCTC              
AvaII         307  GGACC                G^G[AT]CC
             1920  GGACC               
BciVI        1134  GTATCC               GTATCC(6/5)
             1255  GTATCC              
BsaJI          26  CCTTGG               C^C[ACGT][ACGT]GG
              804  CCTAGG              
BsiHKAI       294  GAGCTC               G[AT]GC[AT]^C
             1270  GAGCTC              
BsrDI         398  CATTGC               GCAATG(2/0)
              976  CATTGC              
EcoT14I        26  CCTTGG               C^C[AT][AT]GG
              804  CCTAGG              
EcoT22I       913  ATGCAT               ATGCA^T
             1430  ATGCAT              
NsiI          913  ATGCAT               ATGCA^T
             1430  ATGCAT              
SacI          294  GAGCTC               GAGCT^C
             1270  GAGCTC              
StyI           26  CCTTGG               C^C[AT][AT]GG
              804  CCTAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BanII       BbvI        BmyI        BpmI        BsiYI       
BslI        BsmAI       BsmI        Bsp1286I    BsrI        
BstF5I      Cfr13I      DdeI        DraI        Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeIII      MboII       MnlI        MseI        MslI        
MwoI        NlaIII      NspI        PleI        RsaI        
Sau96I      SfaNI       TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI