Restriction map for
pFN21AE3405
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BciVI BclI
BfaI BfrI BlnI BsaAI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FbaI FspI HindIII HpaI
KpnI KspI MaeI MaeIII MamI
MfeI MluI MroI MseI MslI
MunI MvnI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I Van91I XbaI
XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 480 GACGTC GACGT^C
AccI 157 GTCGAC GT^[AC][GT]AC
AfaI 42 GTAC GT^AC
AlwI 183 GATCC GGATC(4/5)
AocI 519 CCTGAGG CC^T[ACGT]AGG
Aor51HI 107 AGCGCT AGC^GCT
AvaII 27 GGACC G^G[AT]CC
BalI 527 TGGCCA TGG^CCA
BbeI 121 GGCGCC GGCGC^C
BbsI 171 GAAGAC GAAGAC(2/6)
BcgI 602 GCAAGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 271 GCCAATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 209 AGATCT A^GATCT
BlpI 448 GCTGAGC GC^T[ACGT]AGC
BpmI 178 CTGGAG CTGGAG(16/14)
Bpu1102I 448 GCTGAGC GC^T[ACGT]AGC
BpuAI 171 GAAGAC GAAGAC(2/6)
BsaI 420 GGTCTC GGTCTC(1/5)
BsiEI 221 CGGCCG CG[GA][CT]^CG
BsmBI 478 GAGACG CGTCTC(1/5)
BsrBI 232 GAGCGG CCGCTC(-3/-3)
BsrI 332 ACTGG ACTGG(1/-1)
BstAPI 89 GCAAGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 519 CCTGAGG CC^T[ACGT]AGG
CelII 448 GCTGAGC GC^T[ACGT]AGC
DsaI 530 CCACGG C^C[GA][CT]GG
EagI 221 CGGCCG C^GGCCG
EarI 573 GAAGAG CTCTTC(1/4)
EclXI 221 CGGCCG C^GGCCG
Eco47III 107 AGCGCT AGC^GCT
Eco52I 221 CGGCCG C^GGCCG
Eco81I 519 CCTGAGG CC^T[ACGT]AGG
EcoNI 515 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 222 GGCCGGCC GGCCGG^CC
HincII 157 GTCGAC GT[CT]^[GA]AC
HindII 157 GTCGAC GT[CT]^[GA]AC
HinfI 493 GACTC G^A[ACGT]TC
KasI 121 GGCGCC G^GCGCC
Ksp632I 573 GAAGAG CTCTTC(1/4)
MluNI 527 TGGCCA TGG^CCA
MscI 527 TGGCCA TGG^CCA
NaeI 223 GCCGGC GCC^GGC
NarI 121 GGCGCC GG^CGCC
NgoMIV 223 GCCGGC G^CCGGC
PaeR7I 495 CTCGAG C^TCGAG
PleI 493 GACTC GAGTC(4/5)
PpuMI 26 GGGACCC [GA]G^G[AT]CC[CT]
PstI 205 CTGCAG CTGCA^G
RsaI 42 GTAC GT^AC
SalI 157 GTCGAC G^TCGAC
SapI 573 GAAGAGC GCTCTTC(1/4)
SfcI 205 CTGCAG C^T[GA][CT]AG
SfoI 121 GGCGCC GGC^GCC
SmaI 21 CCCGGG CCC^GGG
SmlI 495 CTCGAG C^T[CT][GA]AG
StuI 512 AGGCCT AGG^CCT
XcmI 167 CCATGAAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 495 CTCGAG C^TCGAG
XmaCI 21 CCCGGG C^CCGGG
XmaI 21 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 376 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
439 CAGAGCCTG
ApaI 12 GGGCCC GGGCC^C
555 GGGCCC
AvaI 21 CCCGGG C^[CT]CG[GA]G
495 CTCGAG
BanI 121 GGCGCC G^G[CT][GA]CC
324 GGTGCC
BbvCI 402 CCTCAGC CCTCAGC(-5/-2)
430 GCTGAGG
BseRI 306 GAGGAG GAGGAG(10/8)
508 GAGGAG
BsgI 103 GTGCAG GTGCAG(16/14)
373 GTGCAG
BsmAI 478 GAGAC GTCTC(1/5)
421 GTCTC
BsmFI 26 GGGAC GGGAC(10/14)
215 GGGAC
BsoBI 21 CCCGGG C^[CT]CG[GA]G
495 CTCGAG
BsrFI 118 ACCGGC [GA]^CCGG[CT]
223 GCCGGC
BstYI 182 AGATCC [GA]^GATC[CT]
209 AGATCT
Cfr10I 118 ACCGGC [GA]^CCGG[CT]
223 GCCGGC
EcoT14I 425 CCTTGG C^C[AT][AT]GG
488 CCAAGG
MboII 171 GAAGA GAAGA(8/7)
573 GAAGA
MflI 182 AGATCC [GA]^GATC[CT]
209 AGATCT
MspA1I 376 CAGCTG C[AC]G^C[GT]G
592 CAGCTG
NlaIII 168 CATG CATG^
543 CATG
PvuII 376 CAGCTG CAG^CTG
592 CAGCTG
SacI 309 GAGCTC GAGCT^C
535 GAGCTC
StyI 425 CCTTGG C^C[AT][AT]GG
488 CCAAGG
TaqI 158 TCGA T^CGA
496 TCGA
TspRI 267 CAGTG CA[GC]TG[ACGT][ACGT]^
331 CACTG
TthHB8I 158 TCGA T^CGA
496 TCGA
XhoII 182 AGATCC [GA]^GATC[CT]
209 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcyI AluI AspHI BanII
BbvI BcnI BmyI BsaHI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeII
MboI MnlI MspI MvaI MwoI
NciI NdeII NlaIV Sau3AI Sau96I
ScrFI TseI