Restriction map for
pFN21AE6487
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BsrBI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MunI MvnI NarI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 1066 AAATTC [GA]^AATT[CT]
AflIII 1025 ACATGT A^C[GA][CT]GT
Alw44I 1034 GTGCAC G^TGCAC
AlwNI 1306 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 122 GGGCCC GGGCC^C
ApaLI 1034 GTGCAC G^TGCAC
ApoI 1066 AAATTC [GA]^AATT[CT]
Asp700I 1062 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1314 GGATCC G^GATCC
BcnI 17 CCCGG CC^[GC]GG
BfaI 170 CTAG C^TAG
Bsp1407I 1408 TGTACA T^GTACA
BspLU11I 1025 ACATGT A^CATGT
BsrFI 247 GCCGGC [GA]^CCGG[CT]
BsrGI 1408 TGTACA T^GTACA
BstAPI 583 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 204 GGTGACC G^GT[ACGT]ACC
BstPI 204 GGTGACC G^GT[ACGT]ACC
CfoI 493 GCGC GCG^C
Cfr10I 247 GCCGGC [GA]^CCGG[CT]
DrdI 180 GACCGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 204 GGTGACC G^GT[ACGT]ACC
HhaI 493 GCGC GCG^C
HinP1I 493 GCGC G^CGC
HincII 643 GTCAAC GT[CT]^[GA]AC
HindII 643 GTCAAC GT[CT]^[GA]AC
MaeI 170 CTAG C^TAG
MaeII 1099 ACGT A^CGT
MseI 1462 TTAA T^TAA
NaeI 247 GCCGGC GCC^GGC
NciI 17 CCCGG CC^[GC]GG
NcoI 875 CCATGG C^CATGG
NdeI 963 CATATG CA^TATG
NgoMIV 247 GCCGGC G^CCGGC
PflMI 1305 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 113 GAGCTC GAGCT^C
SexAI 1181 ACCTGGT A^CC[AT]GGT
SphI 251 GCATGC GCATG^C
SspBI 1408 TGTACA T^GTACA
TfiI 1007 GAATC G^A[AT]TC
Tru9I 1462 TTAA T^TAA
Van91I 1305 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 704 CCATTCTGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1062 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 759 GACGCC G[GA]^CG[CT]C
1042 GGCGTC
BalI 872 TGGCCA TGG^CCA
989 TGGCCA
BanI 315 GGTGCC G^G[CT][GA]CC
1301 GGCACC
BbvCI 75 CCTCAGC CCTCAGC(-5/-2)
774 CCTCAGC
BsaHI 759 GACGCC G[GA]^CG[CT]C
1042 GGCGTC
BstXI 149 CCAAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
461 CCACTGTCGTGG
BstYI 839 AGATCC [GA]^GATC[CT]
1314 GGATCC
DsaI 875 CCATGG C^C[GA][CT]GG
1376 CCGTGG
EaeI 872 TGGCCA [CT]^GGCC[GA]
989 TGGCCA
EcoT14I 536 CCAAGG C^C[AT][AT]GG
875 CCATGG
HapII 18 CCGG C^CGG
248 CCGG
HgaI 759 GACGC GACGC(5/10)
1043 GCGTC
Hin1I 759 GACGCC G[GA]^CG[CT]C
1042 GGCGTC
HpaII 18 CCGG C^CGG
248 CCGG
MflI 839 AGATCC [GA]^GATC[CT]
1314 GGATCC
MluNI 872 TGGCCA TGG^CCA
989 TGGCCA
MscI 872 TGGCCA TGG^CCA
989 TGGCCA
MslI 447 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
462 CACTGTCGTG
MspI 18 CCGG C^CGG
248 CCGG
NspI 251 GCATGC [GA]CATG^[CT]
1025 ACATGT
PaeR7I 515 CTCGAG C^TCGAG
1124 CTCGAG
PpuMI 803 GGGTCCT [GA]G^G[AT]CC[CT]
1355 GGGACCT
PvuII 36 CAGCTG CAG^CTG
377 CAGCTG
SfaNI 361 GCATC GCATC(5/9)
1331 GCATC
SfcI 95 CTGTAG C^T[GA][CT]AG
732 CTACAG
StyI 536 CCAAGG C^C[AT][AT]GG
875 CCATGG
XhoI 515 CTCGAG C^TCGAG
1124 CTCGAG
XhoII 839 AGATCC [GA]^GATC[CT]
1314 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanII BbvI BmyI
BpmI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoNI EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MnlI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SmlI StuI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I