Restriction map for
pFN21AE6071
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsoBI Bsp1286I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HindIII HpaII
KasI KpnI KspI MaeII MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
AlwI 571 GATCC GGATC(4/5)
Asp700I 269 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 778 GGACC G^G[AT]CC
BalI 371 TGGCCA TGG^CCA
BbvI 25 GCAGC GCAGC(8/12)
BfaI 5 CTAG C^TAG
BsaBI 594 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 742 GTCTC GTCTC(1/5)
BsrDI 630 CATTGC GCAATG(2/0)
BsrI 808 CCAGT ACTGG(1/-1)
BssKI 195 CCTGG ^CC[ACGT]GG
BstEII 108 GGTCACC G^GT[ACGT]ACC
BstNI 195 CCTGG CC^[AT]GG
BstPI 108 GGTCACC G^GT[ACGT]ACC
BstXI 98 CCATCTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 570 AGATCC [GA]^GATC[CT]
DraII 439 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 113 CCATGG C^C[GA][CT]GG
EaeI 371 TGGCCA [CT]^GGCC[GA]
EarI 698 GAAGAG CTCTTC(1/4)
EcoO109I 439 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 108 GGTCACC G^GT[ACGT]ACC
EcoRI 31 GAATTC G^AATTC
EcoRII 195 CCTGG ^CC[AT]GG
Fnu4HI 25 GCAGC GC^[ACGT]GC
HincII 495 GTTAAC GT[CT]^[GA]AC
HindII 495 GTTAAC GT[CT]^[GA]AC
HpaI 495 GTTAAC GTT^AAC
ItaI 25 GCAGC GC^[ACGT]GC
Ksp632I 698 GAAGAG CTCTTC(1/4)
MaeI 5 CTAG C^TAG
MamI 594 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 570 AGATCC [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MspA1I 26 CAGCTG C[AC]G^C[GT]G
MvaI 195 CCTGG CC^[AT]GG
NcoI 113 CCATGG C^CATGG
NdeI 375 CATATG CA^TATG
NlaIV 439 GGGGCC GG[ACGT]^[ACGT]CC
PleI 557 GACTC GAGTC(4/5)
PvuII 26 CAGCTG CAG^CTG
ScrFI 195 CCTGG CC^[ACGT]GG
TseI 25 GCAGC G^C[AT]GC
Tsp45I 109 GTCAC ^GT[GC]AC
XhoII 570 AGATCC [GA]^GATC[CT]
XmnI 269 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AseI 141 ATTAAT AT^TAAT
580 ATTAAT
AsnI 141 ATTAAT AT^TAAT
580 ATTAAT
BsaJI 113 CCATGG C^C[ACGT][ACGT]GG
689 CCAAGG
BsiYI 113 CCATGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
363 CCGCTATGTGG
BslI 113 CCATGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
363 CCGCTATGTGG
BsmI 422 GCATTC GAATGC(1/-1)
462 GCATTC
Bsp1407I 176 TGTACA T^GTACA
565 TGTACA
BspMI 458 ACCTGC ACCTGC(4/8)
506 GCAGGT
BsrGI 176 TGTACA T^GTACA
565 TGTACA
Cfr13I 440 GGGCC G^G[ACGT]CC
778 GGACC
DpnII 571 GATC ^GATC
645 GATC
EcoT14I 113 CCATGG C^C[AT][AT]GG
689 CCAAGG
HphI 110 TCACC GGTGA(8/7)
522 TCACC
MaeIII 109 GTCAC ^GT[ACGT]AC
229 GTTAC
MboI 571 GATC ^GATC
645 GATC
NdeII 571 GATC ^GATC
645 GATC
PshBI 141 ATTAAT AT^TAAT
580 ATTAAT
PstI 388 CTGCAG CTGCA^G
504 CTGCAG
Sau3AI 571 GATC ^GATC
645 GATC
Sau96I 440 GGGCC G^G[ACGT]CC
778 GGACC
SfaNI 659 GCATC GCATC(5/9)
724 GCATC
SspBI 176 TGTACA T^GTACA
565 TGTACA
SspI 747 AATATT AAT^ATT
816 AATATT
StyI 113 CCATGG C^C[AT][AT]GG
689 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbsI
BpuAI BstF5I DdeI FokI HaeIII
HgaI HinfI MboII MnlI MseI
MslI MwoI NlaIII RsaI SfcI
TfiI Tru9I Tsp509I TspRI