Restriction map for
pFN21AE5985
##################################
# NO CUT ENZYMES ( 197 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BsrI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRII EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI ItaI KasI KpnI KspI
MaeI MaeII MamI MflI MluI
MluNI MroI MscI MspA1I MvaI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TseI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 21 enzymes) #
###################################
AciI 793 CCGC CCGC(-3/-1)
AcsI 192 GAATTT [GA]^AATT[CT]
ApaI 780 GGGCCC GGGCC^C
ApoI 192 GAATTT [GA]^AATT[CT]
BsaJI 186 CCTTGG C^C[ACGT][ACGT]GG
Cac8I 795 GCAAGC GC[ACGT]^[ACGT]GC
DpnII 361 GATC ^GATC
DraII 780 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 780 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 186 CCTTGG C^C[AT][AT]GG
HapII 426 CCGG C^CGG
HpaII 426 CCGG C^CGG
MboI 361 GATC ^GATC
MfeI 314 CAATTG C^AATTG
MspI 426 CCGG C^CGG
MunI 314 CAATTG C^AATTG
MwoI 346 GCAACAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 361 GATC ^GATC
Sau3AI 361 GATC ^GATC
SfcI 867 CTACAG C^T[GA][CT]AG
StyI 186 CCTTGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AseI 432 ATTAAT AT^TAAT
580 ATTAAT
AsnI 432 ATTAAT AT^TAAT
580 ATTAAT
Asp700I 246 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
331 GAATGTTTTC
BanII 725 GAGCCC G[GA]GC[CT]^C
780 GGGCCC
BmyI 725 GAGCCC G[AGT]GC[ACT]^C
780 GGGCCC
BsiYI 78 CCTGTTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
113 CCATCATGGGG
BslI 78 CCTGTTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
113 CCATCATGGGG
BsmI 286 GAATGC GAATGC(1/-1)
88 GCATTC
Bsp1286I 725 GAGCCC G[AGT]GC[ACT]^C
780 GGGCCC
BsrDI 20 CATTGC GCAATG(2/0)
309 CATTGC
EcoRV 216 GATATC GAT^ATC
273 GATATC
HaeIII 757 GGCC GG^CC
781 GGCC
HinfI 123 GAATC G^A[ACGT]TC
571 GATTC
HphI 110 TCACC GGTGA(8/7)
159 TCACC
MslI 111 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
903 CATAAAAATG
NdeI 356 CATATG CA^TATG
375 CATATG
PshBI 432 ATTAAT AT^TAAT
580 ATTAAT
TfiI 123 GAATC G^A[AT]TC
571 GATTC
XmnI 246 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
331 GAATGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AfaI AluI BstF5I Cfr13I DdeI
EarI FokI Ksp632I MaeIII MboII
MnlI MseI NlaIII NlaIV RsaI
Sau96I SfaNI Tru9I Tsp45I Tsp509I
TspRI