Restriction map for
pFN21AE2437
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraIII DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
HinP1I HindIII HpaI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI SspI StuI SwaI
Tru9I Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 395 GTAGAC GT^[AC][GT]AC
AccII 941 CGCG CG^CG
AcsI 29 GAATTT [GA]^AATT[CT]
AcyI 162 GACGCC G[GA]^CG[CT]C
AlwNI 446 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 29 GAATTT [GA]^AATT[CT]
AvaI 853 CCCGGG C^[CT]CG[GA]G
BanI 1117 GGCACC G^G[CT][GA]CC
BbsI 692 GAAGAC GAAGAC(2/6)
BfaI 292 CTAG C^TAG
BglI 746 GCCCACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1030 AGATCT A^GATCT
BpuAI 692 GAAGAC GAAGAC(2/6)
BsaHI 162 GACGCC G[GA]^CG[CT]C
BsoBI 853 CCCGGG C^[CT]CG[GA]G
BsrFI 1226 ACCGGC [GA]^CCGG[CT]
BstUI 941 CGCG CG^CG
Cfr10I 1226 ACCGGC [GA]^CCGG[CT]
DrdI 261 GACCTCGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 939 CCCGC CCCGC(4/6)
HgaI 162 GACGC GACGC(5/10)
Hin1I 162 GACGCC G[GA]^CG[CT]C
MaeI 292 CTAG C^TAG
MvnI 941 CGCG CG^CG
NdeI 1301 CATATG CA^TATG
PflMI 434 CCATCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1339 GACTC GAGTC(4/5)
PshAI 577 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1072 CAGCTG CAG^CTG
SbfI 808 CCTGCAGG CCTGCA^GG
ScaI 1325 AGTACT AGT^ACT
SfaNI 1190 GCATC GCATC(5/9)
SmaI 853 CCCGGG CCC^GGG
SmlI 982 CTTGAG C^T[CT][GA]AG
Sse8387I 808 CCTGCAGG CCTGCA^GG
TaqI 1337 TCGA T^CGA
Tsp509I 30 AATT ^AATT
TthHB8I 1337 TCGA T^CGA
Van91I 434 CCATCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 853 CCCGGG C^CCGGG
XmaI 853 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 1038 GTAC GT^AC
1326 GTAC
BalI 138 TGGCCA TGG^CCA
442 TGGCCA
BamHI 538 GGATCC G^GATCC
556 GGATCC
BpmI 860 CTCCAG CTGGAG(16/14)
256 CTGGAG
BspMI 807 ACCTGC ACCTGC(4/8)
1310 ACCTGC
BsrDI 1008 CATTGC GCAATG(2/0)
1314 GCAATG
DdeI 65 CTCAG C^T[ACGT]AG
99 CTCAG
EcoT14I 524 CCAAGG C^C[AT][AT]GG
1053 CCTTGG
HincII 773 GTCAAC GT[CT]^[GA]AC
964 GTCAAC
HindII 773 GTCAAC GT[CT]^[GA]AC
964 GTCAAC
MluNI 138 TGGCCA TGG^CCA
442 TGGCCA
MscI 138 TGGCCA TGG^CCA
442 TGGCCA
MslI 1225 CACCGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1231 CATGAGGGTG
MspA1I 1072 CAGCTG C[AC]G^C[GT]G
1194 CCGCTG
RsaI 1038 GTAC GT^AC
1326 GTAC
StyI 524 CCAAGG C^C[AT][AT]GG
1053 CCTTGG
TspRI 179 CACTG CA[GC]TG[ACGT][ACGT]^
1101 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AluI AlwI ApaI AvaII
BanII BbvI BcnI BmyI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstEII
BstF5I BstNI BstPI BstYI Cac8I
Cfr13I DpnII DraII EaeI EcoO109I
EcoO65I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PpuMI PstI
Sau3AI Sau96I ScrFI SfcI SphI
TfiI TseI Tsp45I XhoII