Restriction map for
pFN21AE0088
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII AccIII AclI AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII BbvCI BglII BlpI Bpu1102I
BsaI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI Ksp632I KspI
MfeI MluI MroI MunI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SphI Sse8387I StuI
SwaI Tth111I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 1639 GGTACC G^GTACC
AccI 554 GTCTAC GT^[AC][GT]AC
AcyI 1061 GGCGCC G[GA]^CG[CT]C
AflII 718 CTTAAG C^TTAAG
AflIII 866 ACGTGT A^C[GA][CT]GT
ApaI 1469 GGGCCC GGGCC^C
Asp718I 1639 GGTACC G^GTACC
AvrII 1605 CCTAGG C^CTAGG
BamHI 2171 GGATCC G^GATCC
BbeI 1061 GGCGCC GGCGC^C
BbrPI 865 CACGTG CAC^GTG
BciVI 2666 GTATCC GTATCC(6/5)
BclI 1088 TGATCA T^GATCA
BfrI 718 CTTAAG C^TTAAG
BglI 1957 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1605 CCTAGG C^CTAGG
BsaBI 1159 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1061 GGCGCC G[GA]^CG[CT]C
BsaWI 1625 ACCGGA [AT]^CCGG[AT]
BsgI 355 GTGCAG GTGCAG(16/14)
BsmBI 2685 GAGACG CGTCTC(1/5)
BsmFI 44 GGGAC GGGAC(10/14)
BsmI 1616 GCATTC GAATGC(1/-1)
FbaI 1088 TGATCA T^GATCA
HaeII 1061 GGCGCC [GA]GCGC^[CT]
HgaI 2235 GACGC GACGC(5/10)
Hin1I 1061 GGCGCC G[GA]^CG[CT]C
KasI 1061 GGCGCC G^GCGCC
KpnI 1639 GGTACC GGTAC^C
MamI 1159 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1061 GGCGCC GG^CGCC
NdeI 2374 CATATG CA^TATG
NspI 286 GCATGT [GA]CATG^[CT]
PmaCI 865 CACGTG CAC^GTG
PmlI 865 CACGTG CAC^GTG
SfoI 1061 GGCGCC GGC^GCC
SmaI 28 CCCGGG CCC^GGG
SpeI 1446 ACTAGT A^CTAGT
XmaCI 28 CCCGGG C^CCGGG
XmaI 28 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 36 enzymes) #
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AccII 278 CGCG CG^CG
2199 CGCG
AcsI 1218 GAATTC [GA]^AATT[CT]
2521 GAATTC
AlwNI 704 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
724 CAGATTCTG
ApoI 1218 GAATTC [GA]^AATT[CT]
2521 GAATTC
BalI 992 TGGCCA TGG^CCA
1286 TGGCCA
BanI 1061 GGCGCC G^G[CT][GA]CC
1639 GGTACC
BbsI 532 GAAGAC GAAGAC(2/6)
2419 GAAGAC
BcgI 11 GCACACTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
368 GCAATGCTCTCG
BpmI 579 CTCCAG CTGGAG(16/14)
1679 CTCCAG
BpuAI 532 GAAGAC GAAGAC(2/6)
2419 GAAGAC
BsaAI 865 CACGTG [CT]AC^GT[GA]
1561 TACGTG
Bsp1407I 983 TGTACA T^GTACA
1337 TGTACA
BspHI 1598 TCATGA T^CATGA
2123 TCATGA
BsrDI 996 CATTGC GCAATG(2/0)
368 GCAATG
BsrGI 983 TGTACA T^GTACA
1337 TGTACA
BstUI 278 CGCG CG^CG
2199 CGCG
CfoI 1062 GCGC GCG^C
2200 GCGC
DsaI 893 CCATGG C^C[GA][CT]GG
1057 CCATGG
EcoRI 1218 GAATTC G^AATTC
2521 GAATTC
HhaI 1062 GCGC GCG^C
2200 GCGC
HinP1I 1062 GCGC G^CGC
2200 GCGC
MluNI 992 TGGCCA TGG^CCA
1286 TGGCCA
MscI 992 TGGCCA TGG^CCA
1286 TGGCCA
MslI 735 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1557 CACCTACGTG
MvnI 278 CGCG CG^CG
2199 CGCG
MwoI 1528 GCTGACCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1957 GCCAATCTGGC
NcoI 893 CCATGG C^CATGG
1057 CCATGG
PstI 904 CTGCAG CTGCA^G
1036 CTGCAG
RcaI 1598 TCATGA T^CATGA
2123 TCATGA
SacI 562 GAGCTC GAGCT^C
1850 GAGCTC
ScaI 105 AGTACT AGT^ACT
752 AGTACT
SspBI 983 TGTACA T^GTACA
1337 TGTACA
SspI 589 AATATT AAT^ATT
2455 AATATT
TaqI 377 TCGA T^CGA
1894 TCGA
TthHB8I 377 TCGA T^CGA
1894 TCGA
XcmI 294 CCAGCTGGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
609 CCAGCTGCCCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
NciI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII