Restriction map for
pFN21AE3349
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp718I AviII AvrII BbeI BbrPI
BcgI BciVI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaWI BseAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BssHII Bst1107I BstBI
BstZ17I CelII ClaI DraI DraIII
Eco47III EcoRI EcoRV FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MroI NarI NcoI
NdeI NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 382 GTCTAC GT^[AC][GT]AC
AcyI 259 GACGCC G[GA]^CG[CT]C
AflIII 966 ACGCGT A^C[GA][CT]GT
AhdI 511 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 694 GTGCAC G^TGCAC
AocI 1500 CCTAAGG CC^T[ACGT]AGG
ApaLI 694 GTGCAC G^TGCAC
Asp700I 2807 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 511 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2699 TGGCCA TGG^CCA
BamHI 1681 GGATCC G^GATCC
BclI 267 TGATCA T^GATCA
BsaBI 976 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 259 GACGCC G[GA]^CG[CT]C
BsaI 2936 GGTCTC GGTCTC(1/5)
BsiEI 985 CGGCCG CG[GA][CT]^CG
BsmBI 555 CGTCTC CGTCTC(1/5)
Bsp1407I 1357 TGTACA T^GTACA
BspMI 2615 ACCTGC ACCTGC(4/8)
BsrGI 1357 TGTACA T^GTACA
BssSI 756 CACGAG CACGAG(-5/-1)
Bsu36I 1500 CCTAAGG CC^T[ACGT]AGG
CpoI 550 CGGTCCG CG^G[AT]CCG
DsaI 2126 CCACGG C^C[GA][CT]GG
EagI 985 CGGCCG C^GGCCG
Eam1105I 511 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 985 CGGCCG C^GGCCG
Eco52I 985 CGGCCG C^GGCCG
Eco81I 1500 CCTAAGG CC^T[ACGT]AGG
EcoT14I 426 CCTTGG C^C[AT][AT]GG
EcoT22I 2070 ATGCAT ATGCA^T
FbaI 267 TGATCA T^GATCA
Hin1I 259 GACGCC G[GA]^CG[CT]C
MamI 976 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 357 CAATTG C^AATTG
MluI 966 ACGCGT A^CGCGT
MluNI 2699 TGGCCA TGG^CCA
MscI 2699 TGGCCA TGG^CCA
MunI 357 CAATTG C^AATTG
NaeI 2528 GCCGGC GCC^GGC
NgoMIV 2528 GCCGGC G^CCGGC
NsiI 2070 ATGCAT ATGCA^T
NspI 3018 ACATGC [GA]CATG^[CT]
PaeR7I 2583 CTCGAG C^TCGAG
PshAI 375 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 550 CGGTCCG CG^G[AT]CCG
SacI 1158 GAGCTC GAGCT^C
SmaI 2915 CCCGGG CCC^GGG
SspBI 1357 TGTACA T^GTACA
StyI 426 CCTTGG C^C[AT][AT]GG
XcmI 1187 CCACCCACATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2583 CTCGAG C^TCGAG
XmaCI 2915 CCCGGG C^CCGGG
XmaI 2915 CCCGGG C^CCGGG
XmnI 2807 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 663 CGCG CG^CG
967 CGCG
AcsI 2740 AAATTT [GA]^AATT[CT]
2977 AAATTC
ApoI 2740 AAATTT [GA]^AATT[CT]
2977 AAATTC
AspHI 694 GTGCAC G[AT]GC[AT]^C
1158 GAGCTC
AspI 2301 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2389 GACATGGTC
AvaI 2583 CTCGAG C^[CT]CG[GA]G
2915 CCCGGG
BbsI 1510 GAAGAC GAAGAC(2/6)
1813 GAAGAC
BbvCI 2548 CCTCAGC CCTCAGC(-5/-2)
1722 GCTGAGG
BfaI 160 CTAG C^TAG
679 CTAG
BglII 1133 AGATCT A^GATCT
2909 AGATCT
BpuAI 1510 GAAGAC GAAGAC(2/6)
1813 GAAGAC
BsgI 583 GTGCAG GTGCAG(16/14)
657 GTGCAG
BsiHKAI 694 GTGCAC G[AT]GC[AT]^C
1158 GAGCTC
BsoBI 2583 CTCGAG C^[CT]CG[GA]G
2915 CCCGGG
BsrFI 2217 GCCGGT [GA]^CCGG[CT]
2528 GCCGGC
BstEII 948 GGTCACC G^GT[ACGT]ACC
1221 GGTCACC
BstPI 948 GGTCACC G^GT[ACGT]ACC
1221 GGTCACC
BstUI 663 CGCG CG^CG
967 CGCG
BstXI 1772 CCACACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2453 CCATGAAGCTGG
Cfr10I 2217 GCCGGT [GA]^CCGG[CT]
2528 GCCGGC
EaeI 985 CGGCCG [CT]^GGCC[GA]
2699 TGGCCA
EcoO65I 948 GGTCACC G^GT[ACGT]ACC
1221 GGTCACC
MaeI 160 CTAG C^TAG
679 CTAG
MvnI 663 CGCG CG^CG
967 CGCG
PflFI 2301 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2389 GACATGGTC
PpuMI 1944 GGGTCCC [GA]G^G[AT]CC[CT]
2444 GGGTCCC
Tth111I 2301 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2389 GACATGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DrdI EarI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII