Restriction map for
pFN21AE6751
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccI AclI AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AviII BbrPI
BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bpu1102I BpuAI
BsaBI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraI DrdI Eam1105I EarI
Eco47III EcoRI EcoRV EcoT22I FbaI
FspI HindIII HpaI Ksp632I MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
XbaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 271 GGTACC G^GTACC
AccIII 653 TCCGGA T^CCGGA
AcsI 940 AAATTC [GA]^AATT[CT]
AflII 460 CTTAAG C^TTAAG
AlwNI 730 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 558 GGGCCC GGGCC^C
ApoI 940 AAATTC [GA]^AATT[CT]
Asp700I 706 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 271 GGTACC G^GTACC
AspI 343 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1072 CCTAGG C^CTAGG
BamHI 1068 GGATCC G^GATCC
BbeI 168 GGCGCC GGCGC^C
BfrI 460 CTTAAG C^TTAAG
BlnI 1072 CCTAGG C^CTAGG
BseAI 653 TCCGGA T^CCGGA
BsmI 794 GCATTC GAATGC(1/-1)
BspEI 653 TCCGGA T^CCGGA
BsrBI 594 GAGCGG CCGCTC(-3/-3)
BssHII 129 GCGCGC G^CGCGC
BstAPI 1274 GCACTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 543 GGTCACC G^GT[ACGT]ACC
BstPI 543 GGTCACC G^GT[ACGT]ACC
BstYI 1068 GGATCC [GA]^GATC[CT]
DraIII 427 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 248 CGGCCG C^GGCCG
EclXI 248 CGGCCG C^GGCCG
Eco52I 248 CGGCCG C^GGCCG
EcoO65I 543 GGTCACC G^GT[ACGT]ACC
FseI 902 GGCCGGCC GGCCGG^CC
HaeII 168 GGCGCC [GA]GCGC^[CT]
HincII 850 GTCAAC GT[CT]^[GA]AC
HindII 850 GTCAAC GT[CT]^[GA]AC
KasI 168 GGCGCC G^GCGCC
KpnI 271 GGTACC GGTAC^C
MflI 1068 GGATCC [GA]^GATC[CT]
MroI 653 TCCGGA T^CCGGA
MslI 795 CATTCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 168 GGCGCC GG^CGCC
NcoI 1474 CCATGG C^CATGG
NspI 1467 GCATGC [GA]CATG^[CT]
PaeR7I 589 CTCGAG C^TCGAG
PflFI 343 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1570 CTGCAG CTGCA^G
PvuII 1431 CAGCTG CAG^CTG
SacI 1648 GAGCTC GAGCT^C
SfoI 168 GGCGCC GGC^GCC
SmaI 805 CCCGGG CCC^GGG
SphI 1467 GCATGC GCATG^C
Tth111I 343 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 589 CTCGAG C^TCGAG
XhoII 1068 GGATCC [GA]^GATC[CT]
XmaCI 805 CCCGGG C^CCGGG
XmaI 805 CCCGGG C^CCGGG
XmnI 706 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwI 1069 GATCC GGATC(4/5)
1068 GGATC
AocI 532 CCTGAGG CC^T[ACGT]AGG
688 CCTCAGG
AvaI 589 CTCGAG C^[CT]CG[GA]G
805 CCCGGG
BalI 1032 TGGCCA TGG^CCA
1358 TGGCCA
BsaAI 157 TACGTG [CT]AC^GT[GA]
718 CACGTA
BsoBI 589 CTCGAG C^[CT]CG[GA]G
805 CCCGGG
BspMI 1279 ACCTGC ACCTGC(4/8)
1374 GCAGGT
BstXI 604 CCAGTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1046 CCAAGAGCTTGG
Bsu36I 532 CCTGAGG CC^T[ACGT]AGG
688 CCTCAGG
DpnII 696 GATC ^GATC
1069 GATC
DraII 237 AGGTCCC [GA]G^G[ACGT]CC[CT]
1707 GGGACCT
Eco81I 532 CCTGAGG CC^T[ACGT]AGG
688 CCTCAGG
EcoO109I 237 AGGTCCC [GA]G^G[ACGT]CC[CT]
1707 GGGACCT
KspI 314 CCGCGG CCGC^GG
468 CCGCGG
MboI 696 GATC ^GATC
1069 GATC
MluNI 1032 TGGCCA TGG^CCA
1358 TGGCCA
MscI 1032 TGGCCA TGG^CCA
1358 TGGCCA
NdeII 696 GATC ^GATC
1069 GATC
PflMI 950 CCATCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1748 CCAGGACTTGG
PpuMI 237 AGGTCCC [GA]G^G[AT]CC[CT]
1707 GGGACCT
SacII 314 CCGCGG CCGC^GG
468 CCGCGG
Sau3AI 696 GATC ^GATC
1069 GATC
SfcI 84 CTACAG C^T[GA][CT]AG
1570 CTGCAG
SmlI 460 CTTAAG C^T[CT][GA]AG
589 CTCGAG
TaqI 590 TCGA T^CGA
1665 TCGA
Tsp509I 867 AATT ^AATT
941 AATT
TthHB8I 590 TCGA T^CGA
1665 TCGA
Van91I 950 CCATCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1748 CCAGGACTTGG
XcmI 661 CCACTCCAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1748 CCAGGACTTGGCTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DsaI EaeI
EcoNI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NgoMIV
NlaIII NlaIV PleI RsaI Sau96I
ScrFI SfaNI StyI TfiI Tru9I
TseI Tsp45I TspRI