Restriction map for
pFN21AE1705
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BclI BcnI BglI BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HindIII HpaII HphI
KasI KpnI KspI MaeII MaeIII
MamI MfeI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tsp45I Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 97 CCGC CCGC(-3/-1)
AfaI 190 GTAC GT^AC
AflII 16 CTTAAG C^TTAAG
AflIII 489 ACATGT A^C[GA][CT]GT
Asp700I 487 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 250 GAAGAC GAAGAC(2/6)
BciVI 84 GTATCC GTATCC(6/5)
BfrI 16 CTTAAG C^TTAAG
BglII 168 AGATCT A^GATCT
BpuAI 250 GAAGAC GAAGAC(2/6)
BsiYI 379 CCTCCCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 379 CCTCCCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 276 GAATGC GAATGC(1/-1)
BspLU11I 489 ACATGT A^CATGT
BspMI 554 ACCTGC ACCTGC(4/8)
BssKI 92 CCAGG ^CC[ACGT]GG
BstNI 92 CCAGG CC^[AT]GG
BstYI 168 AGATCT [GA]^GATC[CT]
DdeI 193 CTTAG C^T[ACGT]AG
DpnII 169 GATC ^GATC
EarI 283 GAAGAG CTCTTC(1/4)
EcoRII 92 CCAGG ^CC[AT]GG
HaeIII 95 GGCC GG^CC
HgaI 253 GACGC GACGC(5/10)
HincII 294 GTTAAC GT[CT]^[GA]AC
HindII 294 GTTAAC GT[CT]^[GA]AC
HpaI 294 GTTAAC GTT^AAC
Ksp632I 283 GAAGAG CTCTTC(1/4)
MboI 169 GATC ^GATC
MflI 168 AGATCT [GA]^GATC[CT]
MslI 597 CATTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 92 CCAGG CC^[AT]GG
MwoI 445 GCAAGAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 169 GATC ^GATC
NlaIV 388 GGGACC GG[ACGT]^[ACGT]CC
PleI 30 GAGTC GAGTC(4/5)
PstI 506 CTGCAG CTGCA^G
RsaI 190 GTAC GT^AC
Sau3AI 169 GATC ^GATC
ScaI 189 AGTACT AGT^ACT
ScrFI 92 CCAGG CC^[ACGT]GG
SfcI 506 CTGCAG C^T[GA][CT]AG
SmlI 16 CTTAAG C^T[CT][GA]AG
TfiI 375 GAATC G^A[AT]TC
TspRI 590 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 65 CCAAGCTACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 168 AGATCT [GA]^GATC[CT]
XmnI 487 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AluI 68 AGCT AG^CT
504 AGCT
AvaII 389 GGACC G^G[AT]CC
606 GGACC
BbvI 106 GCAGC GCAGC(8/12)
505 GCTGC
BsmFI 388 GGGAC GGGAC(10/14)
406 GGGAC
BsrDI 102 CATTGC GCAATG(2/0)
208 GCAATG
BstF5I 48 GGATG GGATG(2/0)
175 GGATG
Cac8I 35 GCCTGC GC[ACGT]^[ACGT]GC
558 GCCAGC
Cfr13I 389 GGACC G^G[ACGT]CC
606 GGACC
FokI 48 GGATG GGATG(9/13)
175 GGATG
HinfI 30 GAGTC G^A[ACGT]TC
375 GAATC
MnlI 379 CCTC CCTC(7/6)
476 CCTC
NspI 109 GCATGT [GA]CATG^[CT]
489 ACATGT
Sau96I 389 GGACC G^G[ACGT]CC
606 GGACC
SfaNI 197 GCATC GCATC(5/9)
562 GCATC
SspI 264 AATATT AAT^ATT
301 AATATT
TaqI 359 TCGA T^CGA
546 TCGA
TseI 106 GCAGC G^C[AT]GC
505 GCTGC
Tsp509I 369 AATT ^AATT
612 AATT
TthHB8I 359 TCGA T^CGA
546 TCGA
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
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BfaI Fnu4HI ItaI MaeI MboII
MseI NlaIII Tru9I