Restriction map for
pFN21AE5640
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AgeI Alw44I AlwNI ApaI ApaLI
AscI AseI AsnI AspI AviII
AvrII BalI BbeI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsiWI BsmBI Bsp1407I BspHI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII CpoI
DraI DraIII DrdI DsaI EcoO65I
EcoRI EcoT14I EcoT22I FseI FspI
Hin1I HpaI KasI KspI MaeI
MamI MfeI MluI MluNI MscI
MspA1I MunI NarI NcoI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 649 GGTACC G^GTACC
AccIII 746 TCCGGA T^CCGGA
AcsI 864 AAATTT [GA]^AATT[CT]
AflIII 876 ACATGT A^C[GA][CT]GT
AhdI 961 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 112 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1316 AGCGCT AGC^GCT
ApoI 864 AAATTT [GA]^AATT[CT]
Asp718I 649 GGTACC G^GTACC
AspEI 961 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 123 GAGCTC G[AT]GC[AT]^C
BamHI 1544 GGATCC G^GATCC
BanI 649 GGTACC G^G[CT][GA]CC
BbrPI 1549 CACGTG CAC^GTG
BclI 1622 TGATCA T^GATCA
BseAI 746 TCCGGA T^CCGGA
BsgI 988 CTGCAC GTGCAG(16/14)
BsiHKAI 123 GAGCTC G[AT]GC[AT]^C
BsmI 981 GAATGC GAATGC(1/-1)
BspDI 544 ATCGAT AT^CGAT
BspEI 746 TCCGGA T^CCGGA
BspLU11I 876 ACATGT A^CATGT
BspMI 659 ACCTGC ACCTGC(4/8)
BsrDI 1497 CATTGC GCAATG(2/0)
BsrI 471 CCAGT ACTGG(1/-1)
BstXI 822 CCAGCGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 112 CCTGAGG CC^T[ACGT]AGG
ClaI 544 ATCGAT AT^CGAT
DraII 182 AGGCCCC [GA]G^G[ACGT]CC[CT]
EagI 6 CGGCCG C^GGCCG
Eam1105I 961 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 6 CGGCCG C^GGCCG
Eco47III 1316 AGCGCT AGC^GCT
Eco52I 6 CGGCCG C^GGCCG
Eco81I 112 CCTGAGG CC^T[ACGT]AGG
EcoNI 1197 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 182 AGGCCCC [GA]G^G[ACGT]CC[CT]
FbaI 1622 TGATCA T^GATCA
HaeII 1316 AGCGCT [GA]GCGC^[CT]
HincII 958 GTTGAC GT[CT]^[GA]AC
HindII 958 GTTGAC GT[CT]^[GA]AC
HindIII 1075 AAGCTT A^AGCTT
KpnI 649 GGTACC GGTAC^C
MroI 746 TCCGGA T^CCGGA
MseI 332 TTAA T^TAA
NaeI 165 GCCGGC GCC^GGC
NdeI 641 CATATG CA^TATG
NgoMIV 165 GCCGGC G^CCGGC
NspI 876 ACATGT [GA]CATG^[CT]
PmaCI 1549 CACGTG CAC^GTG
PmlI 1549 CACGTG CAC^GTG
SacI 123 GAGCTC GAGCT^C
ScaI 422 AGTACT AGT^ACT
SmlI 508 CTCAAG C^T[CT][GA]AG
Tru9I 332 TTAA T^TAA
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# TWO CUT ENZYMES ( 24 enzymes) #
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Asp700I 1314 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1377 GAACAACTTC
AvaI 128 CCCGGG C^[CT]CG[GA]G
635 CCCGGG
BanII 123 GAGCTC G[GA]GC[CT]^C
1533 GGGCTC
BpmI 794 CTCCAG CTGGAG(16/14)
1594 CTCCAG
BsaAI 567 CACGTA [CT]AC^GT[GA]
1549 CACGTG
BseRI 740 CTCCTC GAGGAG(10/8)
300 GAGGAG
BsiEI 6 CGGCCG CG[GA][CT]^CG
75 CGGTCG
BsoBI 128 CCCGGG C^[CT]CG[GA]G
635 CCCGGG
BsrBI 103 GAGCGG CCGCTC(-3/-3)
253 GAGCGG
BsrFI 8 GCCGGT [GA]^CCGG[CT]
165 GCCGGC
BssHII 173 GCGCGC G^CGCGC
175 GCGCGC
Cfr10I 8 GCCGGT [GA]^CCGG[CT]
165 GCCGGC
EaeI 6 CGGCCG [CT]^GGCC[GA]
276 TGGCCG
EarI 1243 CTCTTC CTCTTC(1/4)
1656 GAAGAG
EcoRV 562 GATATC GAT^ATC
802 GATATC
HgaI 1173 GACGC GACGC(5/10)
80 GCGTC
Ksp632I 1243 CTCTTC CTCTTC(1/4)
1656 GAAGAG
MaeIII 1033 GTGAC ^GT[ACGT]AC
1600 GTGAC
SfaNI 894 GATGC GCATC(5/9)
1271 GCATC
SmaI 128 CCCGGG CCC^GGG
635 CCCGGG
Tsp45I 1033 GTGAC ^GT[GC]AC
1600 GTGAC
XmaCI 128 CCCGGG C^CCGGG
635 CCCGGG
XmaI 128 CCCGGG C^CCGGG
635 CCCGGG
XmnI 1314 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1377 GAACAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BbsI BbvI BcnI BmyI
BpuAI BsaJI BsaWI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MflI MnlI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI TaqI
TfiI TseI Tsp509I TspRI TthHB8I
XhoII