Restriction map for
pFN21AE3411
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BbvI
BcgI BciVI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV FauI Fnu4HI
FseI FspI HaeII HapII HhaI
HinP1I HincII HindII HindIII HpaI
HpaII HphI ItaI KasI Ksp632I
KspI MfeI MflI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SpeI SphI
Sse8387I SspBI StuI SwaI TseI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 494 GGTACC G^GTACC
AccI 713 GTATAC GT^[AC][GT]AC
AciI 934 CCGC CCGC(-3/-1)
AcyI 917 GACGCC G[GA]^CG[CT]C
AlwI 121 GATCC GGATC(4/5)
AlwNI 592 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 657 ATTAAT AT^TAAT
AsnI 657 ATTAAT AT^TAAT
Asp700I 388 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 494 GGTACC G^GTACC
AvaII 621 GGACC G^G[AT]CC
BanI 494 GGTACC G^G[CT][GA]CC
BfaI 649 CTAG C^TAG
BglI 594 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 760 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 917 GACGCC G[GA]^CG[CT]C
BsaJI 702 CCATGG C^C[ACGT][ACGT]GG
BspHI 785 TCATGA T^CATGA
BsrBI 934 CCGCTC CCGCTC(-3/-3)
BsrI 28 ACTGG ACTGG(1/-1)
Bst1107I 713 GTATAC GTA^TAC
BstZ17I 713 GTATAC GTA^TAC
Cac8I 590 GCCAGC GC[ACGT]^[ACGT]GC
DsaI 702 CCATGG C^C[GA][CT]GG
EcoNI 195 CCTCATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 702 CCATGG C^C[AT][AT]GG
EcoT22I 226 ATGCAT ATGCA^T
HgaI 917 GACGC GACGC(5/10)
Hin1I 917 GACGCC G[GA]^CG[CT]C
KpnI 494 GGTACC GGTAC^C
MaeI 649 CTAG C^TAG
MamI 760 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 198 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 594 GCCACTGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 702 CCATGG C^CATGG
NlaIV 494 GGTACC GG[ACGT]^[ACGT]CC
NsiI 226 ATGCAT ATGCA^T
PflMI 834 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 657 ATTAAT AT^TAAT
RcaI 785 TCATGA T^CATGA
SmlI 742 CTTGAG C^T[CT][GA]AG
SnaBI 155 TACGTA TAC^GTA
SspI 391 AATATT AAT^ATT
StyI 702 CCATGG C^C[AT][AT]GG
Tsp45I 428 GTGAC ^GT[GC]AC
TspRI 596 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 834 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 388 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 100 AAATTT [GA]^AATT[CT]
446 AAATTC
AfaI 495 GTAC GT^AC
928 GTAC
ApoI 100 AAATTT [GA]^AATT[CT]
446 AAATTC
BbsI 727 GAAGAC GAAGAC(2/6)
914 GAAGAC
BclI 249 TGATCA T^GATCA
305 TGATCA
BpmI 29 CTGGAG CTGGAG(16/14)
841 CTGGAG
BpuAI 727 GAAGAC GAAGAC(2/6)
914 GAAGAC
BsaAI 155 TACGTA [CT]AC^GT[GA]
893 CACGTA
BsiYI 195 CCTCATCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
834 CCAACTCCTGG
BslI 195 CCTCATCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
834 CCAACTCCTGG
BstF5I 178 CATCC GGATG(2/0)
766 CATCC
Cfr13I 621 GGACC G^G[ACGT]CC
873 GGCCC
DraI 261 TTTAAA TTT^AAA
682 TTTAAA
FbaI 249 TGATCA T^GATCA
305 TGATCA
FokI 178 CATCC GGATG(9/13)
766 CATCC
MaeII 156 ACGT A^CGT
894 ACGT
MaeIII 428 GTGAC ^GT[ACGT]AC
689 GTAAC
RsaI 495 GTAC GT^AC
928 GTAC
Sau96I 621 GGACC G^G[ACGT]CC
873 GGCCC
SfaNI 463 GATGC GCATC(5/9)
707 GCATC
TaqI 200 TCGA T^CGA
545 TCGA
TthHB8I 200 TCGA T^CGA
545 TCGA
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI AspHI BanII BmyI BsiHKAI
Bsp1286I BssKI BstNI DdeI DpnII
EcoRII HaeIII HinfI MboI MboII
MnlI MseI MvaI NdeII NlaIII
SacI Sau3AI ScrFI TfiI Tru9I
Tsp509I