Restriction map for
pFN21AE4595
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Aor51HI AscI AseI AsnI AviII
AvrII BcgI BciVI BclI BfrI
BglII BlnI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eco47III EcoT22I FbaI FseI
FspI HindIII HpaI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 31 enzymes) #
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Acc65I 245 GGTACC G^GTACC
AccI 3005 GTCTAC GT^[AC][GT]AC
AcsI 277 GAATTC [GA]^AATT[CT]
AhdI 722 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2206 GTGCAC G^TGCAC
AocI 3423 CCTGAGG CC^T[ACGT]AGG
ApaLI 2206 GTGCAC G^TGCAC
ApoI 277 GAATTC [GA]^AATT[CT]
Asp700I 579 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 245 GGTACC G^GTACC
AspEI 722 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BsiEI 339 CGGCCG CG[GA][CT]^CG
BsmBI 1679 GAGACG CGTCTC(1/5)
Bsp1407I 2758 TGTACA T^GTACA
BsrGI 2758 TGTACA T^GTACA
BssSI 364 CTCGTG CACGAG(-5/-1)
BstAPI 1137 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3423 CCTGAGG CC^T[ACGT]AGG
DrdI 3266 GACTCAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 339 CGGCCG C^GGCCG
Eam1105I 722 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 339 CGGCCG C^GGCCG
Eco52I 339 CGGCCG C^GGCCG
Eco81I 3423 CCTGAGG CC^T[ACGT]AGG
EcoRI 277 GAATTC G^AATTC
EcoRV 2376 GATATC GAT^ATC
KpnI 245 GGTACC GGTAC^C
SexAI 3653 ACCAGGT A^CC[AT]GGT
SspBI 2758 TGTACA T^GTACA
Tsp509I 278 AATT ^AATT
XmnI 579 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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BamHI 227 GGATCC G^GATCC
3633 GGATCC
BbeI 2396 GGCGCC GGCGC^C
2466 GGCGCC
BbrPI 760 CACGTG CAC^GTG
1578 CACGTG
BlpI 942 GCTCAGC GC^T[ACGT]AGC
1596 GCTCAGC
Bpu1102I 942 GCTCAGC GC^T[ACGT]AGC
1596 GCTCAGC
BsaBI 1040 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2290 GATGCCAATC
BsaI 1279 GAGACC GGTCTC(1/5)
3109 GGTCTC
BspLU11I 1246 ACATGT A^CATGT
2755 ACATGT
BsrDI 2715 CATTGC GCAATG(2/0)
186 GCAATG
BsrFI 849 GCCGGT [GA]^CCGG[CT]
1722 GCCGGT
CelII 942 GCTCAGC GC^T[ACGT]AGC
1596 GCTCAGC
Cfr10I 849 GCCGGT [GA]^CCGG[CT]
1722 GCCGGT
HincII 472 GTCAAC GT[CT]^[GA]AC
1726 GTCAAC
HindII 472 GTCAAC GT[CT]^[GA]AC
1726 GTCAAC
KasI 2396 GGCGCC G^GCGCC
2466 GGCGCC
MamI 1040 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2290 GATGCCAATC
MseI 1687 TTAA T^TAA
2839 TTAA
NarI 2396 GGCGCC GG^CGCC
2466 GGCGCC
PmaCI 760 CACGTG CAC^GTG
1578 CACGTG
PmlI 760 CACGTG CAC^GTG
1578 CACGTG
PshAI 1228 GACAACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1695 GACATGCGTC
SfoI 2396 GGCGCC GGC^GCC
2466 GGCGCC
Tru9I 1687 TTAA T^TAA
2839 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI