##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AviII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DdeI        DpnII       
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        Fnu4HI      
FokI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        ItaI        KasI        KpnI        Ksp632I     
KspI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MunI        MvnI        MwoI        
NaeI        NarI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TaqI        TfiI        TseI        
TspRI       Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AfaI          183  GTAC                 GT^AC
AvaI           37  CCCGGG               C^[CT]CG[GA]G
AvrII         363  CCTAGG               C^CTAGG
BfaI          364  CTAG                 C^TAG
BlnI          363  CCTAGG               C^CTAGG
BseRI          64  GAGGAG               GAGGAG(10/8)
BsiYI          54  CCAGGAAATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           54  CCAGGAAATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         212  GTCTC                GTCTC(1/5)
BsmFI          40  GGGAC                GGGAC(10/14)
BsoBI          37  CCCGGG               C^[CT]CG[GA]G
DraII          32  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO109I       32  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       363  CCTAGG               C^C[AT][AT]GG
HapII          38  CCGG                 C^CGG
HindIII       311  AAGCTT               A^AGCTT
HinfI         210  GAGTC                G^A[ACGT]TC
HpaII          38  CCGG                 C^CGG
HphI          397  GGTGA                GGTGA(8/7)
MaeI          364  CTAG                 C^TAG
MboII         306  GAAGA                GAAGA(8/7)
MspI           38  CCGG                 C^CGG
NlaIII        332  CATG                 CATG^
PflMI          54  CCAGGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          210  GAGTC                GAGTC(4/5)
PpuMI          32  AGGACCC              [GA]G^G[AT]CC[CT]
RsaI          183  GTAC                 GT^AC
SfaNI         114  GATGC                GCATC(5/9)
SmaI           37  CCCGGG               CCC^GGG
StuI          413  AGGCCT               AGG^CCT
StyI          363  CCTAGG               C^C[AT][AT]GG
Tsp509I       237  AATT                 ^AATT
Van91I         54  CCAGGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          37  CCCGGG               C^CCGGG
XmaI           37  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AluI          312  AGCT                 AG^CT
              340  AGCT                
AvaII          33  GGACC                G^G[AT]CC
              436  GGACC               
BcnI           37  CCCGG                CC^[GC]GG
               38  CCGGG               
Cfr13I         33  GGACC                G^G[ACGT]CC
              436  GGACC               
HaeIII        361  GGCC                 GG^CC
              414  GGCC                
MseI          239  TTAA                 T^TAA
              380  TTAA                
NciI           37  CCCGG                CC^[GC]GG
               38  CCGGG               
Sau96I         33  GGACC                G^G[ACGT]CC
              436  GGACC               
SfcI            9  CTGTAG               C^T[GA][CT]AG
              143  CTGTAG              
Tru9I         239  TTAA                 T^TAA
              380  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

BsaJI       BssKI       BstNI       EcoRII      MaeIII      
MnlI        MvaI        NlaIV       ScrFI       Tsp45I