Restriction map for
pFN21AE5942
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI BstAPI BstBI BstUI
Bsu36I CelII CfoI Cfr10I Cfr13I
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 60 TCCGGA T^CCGGA
AciI 722 GCGG CCGC(-3/-1)
Asp700I 31 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 57 GGATCC G^GATCC
BciVI 920 GGATAC GTATCC(6/5)
BsaWI 60 TCCGGA [AT]^CCGG[AT]
BseAI 60 TCCGGA T^CCGGA
BsgI 472 CTGCAC GTGCAG(16/14)
BsmAI 664 GTCTC GTCTC(1/5)
BspDI 362 ATCGAT AT^CGAT
BspEI 60 TCCGGA T^CCGGA
BspLU11I 887 ACATGT A^CATGT
BspMI 125 ACCTGC ACCTGC(4/8)
Bst1107I 831 GTATAC GTA^TAC
BstYI 57 GGATCC [GA]^GATC[CT]
BstZ17I 831 GTATAC GTA^TAC
Cac8I 558 GCTCGC GC[ACGT]^[ACGT]GC
ClaI 362 ATCGAT AT^CGAT
DsaI 836 CCGTGG C^C[GA][CT]GG
EarI 79 CTCTTC CTCTTC(1/4)
EcoT14I 200 CCTTGG C^C[AT][AT]GG
FauI 722 GCGGG CCCGC(4/6)
HapII 61 CCGG C^CGG
HindIII 707 AAGCTT A^AGCTT
HinfI 154 GACTC G^A[ACGT]TC
HpaII 61 CCGG C^CGG
Ksp632I 79 CTCTTC CTCTTC(1/4)
MaeII 478 ACGT A^CGT
MboII 80 TCTTC GAAGA(8/7)
MflI 57 GGATCC [GA]^GATC[CT]
MroI 60 TCCGGA T^CCGGA
MseI 733 TTAA T^TAA
MspI 61 CCGG C^CGG
NspI 887 ACATGT [GA]CATG^[CT]
PflMI 685 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 154 GACTC GAGTC(4/5)
PstI 67 CTGCAG CTGCA^G
SalI 205 GTCGAC G^TCGAC
SmlI 553 CTCAAG C^T[CT][GA]AG
StyI 200 CCTTGG C^C[AT][AT]GG
Tru9I 733 TTAA T^TAA
Van91I 685 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 57 GGATCC [GA]^GATC[CT]
XmnI 31 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 205 GTCGAC GT^[AC][GT]AC
831 GTATAC
AflIII 476 ACACGT A^C[GA][CT]GT
887 ACATGT
BalI 107 TGGCCA TGG^CCA
371 TGGCCA
BfaI 541 CTAG C^TAG
917 CTAG
BpmI 26 CTCCAG CTGGAG(16/14)
801 CTCCAG
BsaJI 200 CCTTGG C^C[ACGT][ACGT]GG
836 CCGTGG
BstEII 603 GGTGACC G^GT[ACGT]ACC
840 GGTCACC
BstPI 603 GGTGACC G^GT[ACGT]ACC
840 GGTCACC
EcoO65I 603 GGTGACC G^GT[ACGT]ACC
840 GGTCACC
EcoRI 31 GAATTC G^AATTC
338 GAATTC
HincII 145 GTCAAC GT[CT]^[GA]AC
205 GTCGAC
HindII 145 GTCAAC GT[CT]^[GA]AC
205 GTCGAC
MaeI 541 CTAG C^TAG
917 CTAG
MluNI 107 TGGCCA TGG^CCA
371 TGGCCA
MscI 107 TGGCCA TGG^CCA
371 TGGCCA
MspA1I 493 CAGCTG C[AC]G^C[GT]G
689 CAGCTG
MwoI 234 GCCCAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
429 GCTGCTTCTGC
NlaIV 57 GGATCC GG[ACGT]^[ACGT]CC
122 GGAACC
PvuII 493 CAGCTG CAG^CTG
689 CAGCTG
SfcI 67 CTGCAG C^T[GA][CT]AG
490 CTACAG
TaqI 206 TCGA T^CGA
363 TCGA
TspRI 386 CACTG CA[GC]TG[ACGT][ACGT]^
824 CAGTG
TthHB8I 206 TCGA T^CGA
363 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BseRI BsiYI BslI BspHI
BssKI BstF5I BstNI BstXI DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HphI ItaI MaeIII MboI
MnlI MslI MvaI NdeII NlaIII
RcaI RsaI Sau3AI ScrFI SfaNI
TseI Tsp45I Tsp509I