Restriction map for
pFN21AE4065
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiHKAI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT14I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MseI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StyI SwaI TfiI Tru9I Tth111I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 274 GACGTC GACGT^C
AlwI 486 GGATC GGATC(4/5)
AocI 298 CCTCAGG CC^T[ACGT]AGG
ApaI 120 GGGCCC GGGCC^C
BanII 120 GGGCCC G[GA]GC[CT]^C
BbeI 226 GGCGCC GGCGC^C
BbsI 818 GAAGAC GAAGAC(2/6)
BcnI 207 CCCGG CC^[GC]GG
BfaI 562 CTAG C^TAG
BglI 196 GCCCACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 667 CTCCAG CTGGAG(16/14)
BpuAI 818 GAAGAC GAAGAC(2/6)
BsiWI 702 CGTACG C^GTACG
BsmBI 921 GAGACG CGTCTC(1/5)
BsmI 575 GAATGC GAATGC(1/-1)
Bsp1407I 768 TGTACA T^GTACA
BsrGI 768 TGTACA T^GTACA
BssSI 25 CTCGTG CACGAG(-5/-1)
BstAPI 983 GCAAGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 486 GGATCT [GA]^GATC[CT]
Bsu36I 298 CCTCAGG CC^T[ACGT]AGG
DpnII 487 GATC ^GATC
DraIII 166 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 435 CGGCCA [CT]^GGCC[GA]
Eco81I 298 CCTCAGG CC^T[ACGT]AGG
EcoT22I 937 ATGCAT ATGCA^T
HapII 208 CCGG C^CGG
HgaI 923 GACGC GACGC(5/10)
HpaII 208 CCGG C^CGG
KasI 226 GGCGCC G^GCGCC
MaeI 562 CTAG C^TAG
MboI 487 GATC ^GATC
MflI 486 GGATCT [GA]^GATC[CT]
MslI 606 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 208 CCGG C^CGG
NarI 226 GGCGCC GG^CGCC
NciI 207 CCCGG CC^[GC]GG
NdeII 487 GATC ^GATC
NsiI 937 ATGCAT ATGCA^T
PaeR7I 733 CTCGAG C^TCGAG
PflMI 34 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 282 AGGTCCC [GA]G^G[AT]CC[CT]
Sau3AI 487 GATC ^GATC
SfoI 226 GGCGCC GGC^GCC
SspBI 768 TGTACA T^GTACA
StuI 186 AGGCCT AGG^CCT
Tsp509I 885 AATT ^AATT
Van91I 34 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 995 CCATCTGCCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 733 CTCGAG C^TCGAG
XhoII 486 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 703 GTAC GT^AC
769 GTAC
AvaI 733 CTCGAG C^[CT]CG[GA]G
758 CTCGGG
BanI 61 GGTGCC G^G[CT][GA]CC
226 GGCGCC
BsaI 77 GGTCTC GGTCTC(1/5)
363 GGTCTC
BsmFI 672 GGGAC GGGAC(10/14)
284 GTCCC
BsoBI 733 CTCGAG C^[CT]CG[GA]G
758 CTCGGG
BstXI 458 CCAGGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
605 CCACACAGATGG
CfoI 194 GCGC GCG^C
227 GCGC
EcoNI 68 CCTTTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
294 CCTACCTCAGG
HaeII 193 AGCGCC [GA]GCGC^[CT]
226 GGCGCC
HhaI 194 GCGC GCG^C
227 GCGC
HinP1I 194 GCGC G^CGC
227 GCGC
HphI 791 TCACC GGTGA(8/7)
948 TCACC
MaeII 14 ACGT A^CGT
275 ACGT
MaeIII 277 GTCAC ^GT[ACGT]AC
754 GTGAC
RsaI 703 GTAC GT^AC
769 GTAC
SmlI 507 CTTGAG C^T[CT][GA]AG
733 CTCGAG
Tsp45I 277 GTCAC ^GT[GC]AC
754 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcyI AluI AlwNI AvaII
BbvCI BbvI BmyI BsaHI BsaJI
BseRI BsgI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
Hin1I HinfI ItaI MboII MnlI
MspA1I MvaI MwoI NlaIII NlaIV
PleI PstI Sau96I ScrFI SfaNI
SfcI TaqI TseI TspRI TthHB8I