Restriction map for
pFN21AE2274
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BbeI
BbrPI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII Eam1105I EarI Eco47III Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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ApaI 75 GGGCCC GGGCC^C
Asp700I 787 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 851 CTCGGG C^[CT]CG[GA]G
BamHI 88 GGATCC G^GATCC
BbsI 891 GAAGAC GAAGAC(2/6)
BbvCI 249 CCTCAGC CCTCAGC(-5/-2)
BciVI 315 GGATAC GTATCC(6/5)
BcnI 375 CCCGG CC^[GC]GG
BfaI 595 CTAG C^TAG
BpuAI 891 GAAGAC GAAGAC(2/6)
BsaI 187 GAGACC GGTCTC(1/5)
BsiEI 216 CGGCCG CG[GA][CT]^CG
BsmAI 187 GAGAC GTCTC(1/5)
BsoBI 851 CTCGGG C^[CT]CG[GA]G
BspHI 339 TCATGA T^CATGA
BspMI 396 ACCTGC ACCTGC(4/8)
BstAPI 472 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 631 GGTGACC G^GT[ACGT]ACC
BstPI 631 GGTGACC G^GT[ACGT]ACC
BstYI 88 GGATCC [GA]^GATC[CT]
DrdI 189 GACCAAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 216 CGGCCG C^GGCCG
EclXI 216 CGGCCG C^GGCCG
Eco52I 216 CGGCCG C^GGCCG
EcoNI 776 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 631 GGTGACC G^GT[ACGT]ACC
HapII 376 CCGG C^CGG
HpaII 376 CCGG C^CGG
MaeI 595 CTAG C^TAG
MaeII 385 ACGT A^CGT
MflI 88 GGATCC [GA]^GATC[CT]
MspA1I 538 CCGCTG C[AC]G^C[GT]G
MspI 376 CCGG C^CGG
NciI 375 CCCGG CC^[GC]GG
NheI 594 GCTAGC G^CTAGC
NlaIII 340 CATG CATG^
PstI 721 CTGCAG CTGCA^G
RcaI 339 TCATGA T^CATGA
SfcI 721 CTGCAG C^T[GA][CT]AG
StuI 847 AGGCCT AGG^CCT
TaqI 353 TCGA T^CGA
TthHB8I 353 TCGA T^CGA
XhoII 88 GGATCC [GA]^GATC[CT]
XmnI 787 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BanI 295 GGCACC G^G[CT][GA]CC
478 GGTGCC
BanII 14 GGGCTC G[GA]GC[CT]^C
75 GGGCCC
BmyI 14 GGGCTC G[AGT]GC[ACT]^C
75 GGGCCC
Bsp1286I 14 GGGCTC G[AGT]GC[ACT]^C
75 GGGCCC
BsrI 143 ACTGG ACTGG(1/-1)
302 ACTGG
EaeI 216 CGGCCG [CT]^GGCC[GA]
413 CGGCCA
EcoT14I 10 CCTTGG C^C[AT][AT]GG
742 CCAAGG
FauI 537 CCCGC CCCGC(4/6)
73 GCGGG
HphI 522 GGTGA GGTGA(8/7)
631 GGTGA
MboII 787 GAAGA GAAGA(8/7)
891 GAAGA
PpuMI 32 GGGTCCC [GA]G^G[AT]CC[CT]
127 AGGACCC
StyI 10 CCTTGG C^C[AT][AT]GG
742 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmFI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboI
MnlI MvaI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI SfaNI TfiI
TseI Tsp45I TspRI