Restriction map for
pFN21AE2004
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AvrII BamHI
BbeI BbvCI BcgI BclI BfaI
BfrI BglII BlnI BsaBI BsaHI
BsiWI Bsp1407I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I DraI EagI Eam1105I
EclXI Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MspA1I MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII SacII SbfI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tsp509I
XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1577 GTCGAC GT^[AC][GT]AC
AccIII 377 TCCGGA T^CCGGA
AflIII 239 ACATGT A^C[GA][CT]GT
Aor51HI 500 AGCGCT AGC^GCT
Asp700I 1165 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 177 TGCGCA TGC^GCA
BbsI 1033 GTCTTC GAAGAC(2/6)
BciVI 840 GGATAC GTATCC(6/5)
BpmI 598 CTCCAG CTGGAG(16/14)
BpuAI 1033 GTCTTC GAAGAC(2/6)
BsaI 301 GAGACC GGTCTC(1/5)
BseAI 377 TCCGGA T^CCGGA
BsiEI 833 CGACCG CG[GA][CT]^CG
BsmBI 1136 GAGACG CGTCTC(1/5)
BspDI 589 ATCGAT AT^CGAT
BspEI 377 TCCGGA T^CCGGA
BspHI 524 TCATGA T^CATGA
BspLU11I 239 ACATGT A^CATGT
BspMI 1059 GCAGGT ACCTGC(4/8)
BsrBI 714 CCGCTC CCGCTC(-3/-3)
BstEII 1398 GGTGACC G^GT[ACGT]ACC
BstPI 1398 GGTGACC G^GT[ACGT]ACC
BstXI 521 CCATCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 589 ATCGAT AT^CGAT
CpoI 1703 CGGTCCG CG^G[AT]CCG
DrdI 1677 GACCTACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 500 AGCGCT AGC^GCT
EcoNI 1518 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1398 GGTGACC G^GT[ACGT]ACC
FspI 177 TGCGCA TGC^GCA
HincII 1577 GTCGAC GT[CT]^[GA]AC
HindII 1577 GTCGAC GT[CT]^[GA]AC
MroI 377 TCCGGA T^CCGGA
NaeI 800 GCCGGC GCC^GGC
NgoMIV 800 GCCGGC G^CCGGC
PflMI 1347 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1061 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 524 TCATGA T^CATGA
RsrII 1703 CGGTCCG CG^G[AT]CCG
SalI 1577 GTCGAC G^TCGAC
SapI 348 GCTCTTC GCTCTTC(1/4)
ScaI 1167 AGTACT AGT^ACT
SexAI 1115 ACCAGGT A^CC[AT]GGT
SfiI 406 GGCCGTGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1085 GCATGC GCATG^C
Van91I 1347 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1178 CCATGCTTCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1165 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AlwI 1275 GATCC GGATC(4/5)
536 GGATC
AspI 1144 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1633 GACAATGTC
AvaI 219 CCCGGG C^[CT]CG[GA]G
276 CCCGGG
BalI 117 TGGCCA TGG^CCA
485 TGGCCA
BanI 272 GGCACC G^G[CT][GA]CC
1069 GGTGCC
BbrPI 1267 CACGTG CAC^GTG
1285 CACGTG
BglI 7 GCCATCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
407 GCCGTGAAGGC
BlpI 1257 GCTCAGC GC^T[ACGT]AGC
1605 GCTGAGC
Bpu1102I 1257 GCTCAGC GC^T[ACGT]AGC
1605 GCTGAGC
BsaAI 1267 CACGTG [CT]AC^GT[GA]
1285 CACGTG
BsaWI 377 TCCGGA [AT]^CCGG[AT]
533 ACCGGA
BsmFI 896 GGGAC GGGAC(10/14)
1041 GTCCC
BsmI 3 GAATGC GAATGC(1/-1)
152 GCATTC
BsoBI 219 CCCGGG C^[CT]CG[GA]G
276 CCCGGG
BsrFI 254 GCCGGT [GA]^CCGG[CT]
800 GCCGGC
BstYI 536 GGATCT [GA]^GATC[CT]
1274 AGATCC
CelII 1257 GCTCAGC GC^T[ACGT]AGC
1605 GCTGAGC
Cfr10I 254 GCCGGT [GA]^CCGG[CT]
800 GCCGGC
DraIII 647 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1348 CACCCTGTG
EcoT14I 845 CCTTGG C^C[AT][AT]GG
1565 CCAAGG
HaeII 500 AGCGCT [GA]GCGC^[CT]
609 GGCGCT
MflI 536 GGATCT [GA]^GATC[CT]
1274 AGATCC
MluNI 117 TGGCCA TGG^CCA
485 TGGCCA
MscI 117 TGGCCA TGG^CCA
485 TGGCCA
MseI 311 TTAA T^TAA
1556 TTAA
NspI 239 ACATGT [GA]CATG^[CT]
1085 GCATGC
PflFI 1144 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1633 GACAATGTC
PmaCI 1267 CACGTG CAC^GTG
1285 CACGTG
PmlI 1267 CACGTG CAC^GTG
1285 CACGTG
PstI 266 CTGCAG CTGCA^G
1421 CTGCAG
SacI 34 GAGCTC GAGCT^C
595 GAGCTC
SmaI 219 CCCGGG CCC^GGG
276 CCCGGG
StuI 368 AGGCCT AGG^CCT
1232 AGGCCT
StyI 845 CCTTGG C^C[AT][AT]GG
1565 CCAAGG
TfiI 584 GAATC G^A[AT]TC
883 GAATC
Tru9I 311 TTAA T^TAA
1556 TTAA
TspRI 1404 CAGTG CA[GC]TG[ACGT][ACGT]^
1504 CAGTG
Tth111I 1144 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1633 GACAATGTC
XhoII 536 GGATCT [GA]^GATC[CT]
1274 AGATCC
XmaCI 219 CCCGGG C^CCGGG
276 CCCGGG
XmaI 219 CCCGGG C^CCGGG
276 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaII BanII BbvI BcnI
BmyI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TseI
Tsp45I TthHB8I