Restriction map for
pFN21AE1155
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BamHI BbeI BciVI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRV EcoT22I FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MunI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 37 GTAGAC GT^[AC][GT]AC
AccII 1212 CGCG CG^CG
AfaI 218 GTAC GT^AC
Aor51HI 855 AGCGCT AGC^GCT
BanI 1081 GGTGCC G^G[CT][GA]CC
BbrPI 111 CACGTG CAC^GTG
BbvCI 897 CCTCAGC CCTCAGC(-5/-2)
BcgI 853 CGAGCGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 546 TGATCA T^GATCA
BglI 1255 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1455 CTGGAG CTGGAG(16/14)
BsaAI 111 CACGTG [CT]AC^GT[GA]
BsiEI 966 CGACCG CG[GA][CT]^CG
BsmI 1432 GAATGC GAATGC(1/-1)
BspHI 836 TCATGA T^CATGA
BspLU11I 176 ACATGT A^CATGT
BssSI 1384 CTCGTG CACGAG(-5/-1)
BstUI 1212 CGCG CG^CG
BstXI 522 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 1358 CCGTGG C^C[GA][CT]GG
Eco47III 855 AGCGCT AGC^GCT
EcoRI 160 GAATTC G^AATTC
EcoT14I 182 CCAAGG C^C[AT][AT]GG
FauI 1329 GCGGG CCCGC(4/6)
FbaI 546 TGATCA T^GATCA
FseI 1064 GGCCGGCC GGCCGG^CC
HaeII 855 AGCGCT [GA]GCGC^[CT]
MslI 753 CATCGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 924 CAGCGG C[AC]G^C[GT]G
MvnI 1212 CGCG CG^CG
NspI 176 ACATGT [GA]CATG^[CT]
PflMI 1197 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 111 CACGTG CAC^GTG
PmlI 111 CACGTG CAC^GTG
RcaI 836 TCATGA T^CATGA
RsaI 218 GTAC GT^AC
SacI 1458 GAGCTC GAGCT^C
SbfI 1494 CCTGCAGG CCTGCA^GG
SmlI 1444 CTCAAG C^T[CT][GA]AG
Sse8387I 1494 CCTGCAGG CCTGCA^GG
StyI 182 CCAAGG C^C[AT][AT]GG
TfiI 585 GAATC G^A[AT]TC
Van91I 1197 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 160 GAATTC [GA]^AATT[CT]
1505 AAATTT
AflIII 110 ACACGT A^C[GA][CT]GT
176 ACATGT
AlwNI 281 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
1198 CAGATCCTG
ApoI 160 GAATTC [GA]^AATT[CT]
1505 AAATTT
AspI 399 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
685 GACTACGTC
BanII 610 GGGCTC G[GA]GC[CT]^C
1458 GAGCTC
BbsI 1414 GAAGAC GAAGAC(2/6)
1291 GTCTTC
BpuAI 1414 GAAGAC GAAGAC(2/6)
1291 GTCTTC
BsgI 172 CTGCAC GTGCAG(16/14)
646 CTGCAC
BsmAI 749 GAGAC GTCTC(1/5)
453 GTCTC
BstAPI 432 GCAAATAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
783 GCACATCCTGC
BstYI 282 AGATCC [GA]^GATC[CT]
1199 AGATCC
EarI 372 CTCTTC CTCTTC(1/4)
660 GAAGAG
EcoNI 15 CCTTTACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1265 CCTGCAGCAGG
Ksp632I 372 CTCTTC CTCTTC(1/4)
660 GAAGAG
MflI 282 AGATCC [GA]^GATC[CT]
1199 AGATCC
MseI 326 TTAA T^TAA
1034 TTAA
NaeI 481 GCCGGC GCC^GGC
1065 GCCGGC
NgoMIV 481 GCCGGC G^CCGGC
1065 GCCGGC
PflFI 399 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
685 GACTACGTC
PleI 1477 GACTC GAGTC(4/5)
1372 GAGTC
SfaNI 1227 GATGC GCATC(5/9)
1345 GCATC
TaqI 243 TCGA T^CGA
747 TCGA
Tru9I 326 TTAA T^TAA
1034 TTAA
Tth111I 399 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
685 GACTACGTC
TthHB8I 243 TCGA T^CGA
747 TCGA
XhoII 282 AGATCC [GA]^GATC[CT]
1199 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcyI AluI AlwI AspHI
AvaII BalI BbvI BmyI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MspI MvaI MwoI
NdeII NlaIII NlaIV PpuMI PstI
Sau3AI Sau96I ScrFI SfcI StuI
TseI Tsp45I Tsp509I TspRI