Restriction map for
pFN21AE4068
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# NO CUT ENZYMES ( 125 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AgeI AhdI AocI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tsp509I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 507 GACGTC GACGT^C
AccI 475 GTCTAC GT^[AC][GT]AC
AflIII 539 ACACGT A^C[GA][CT]GT
Alw44I 664 GTGCAC G^TGCAC
AlwI 988 GATCC GGATC(4/5)
Aor51HI 1049 AGCGCT AGC^GCT
ApaLI 664 GTGCAC G^TGCAC
AspI 310 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2 TGGCCA TGG^CCA
BanII 648 GGGCTC G[GA]GC[CT]^C
BbsI 295 GAAGAC GAAGAC(2/6)
BfaI 85 CTAG C^TAG
BglI 551 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 351 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 351 GCTGAGC GC^T[ACGT]AGC
BpuAI 295 GAAGAC GAAGAC(2/6)
BseRI 598 CTCCTC GAGGAG(10/8)
BsmBI 59 GAGACG CGTCTC(1/5)
BspMI 918 GCAGGT ACCTGC(4/8)
BsrBI 804 CCGCTC CCGCTC(-3/-3)
BsrFI 196 ACCGGC [GA]^CCGG[CT]
BstEII 798 GGTCACC G^GT[ACGT]ACC
BstPI 798 GGTCACC G^GT[ACGT]ACC
BstXI 422 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 351 GCTGAGC GC^T[ACGT]AGC
Cfr10I 196 ACCGGC [GA]^CCGG[CT]
DrdI 195 GACCGGCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1049 AGCGCT AGC^GCT
EcoO65I 798 GGTCACC G^GT[ACGT]ACC
EcoT14I 422 CCAAGG C^C[AT][AT]GG
MaeI 85 CTAG C^TAG
MboII 295 GAAGA GAAGA(8/7)
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MseI 328 TTAA T^TAA
NruI 1023 TCGCGA TCG^CGA
PflFI 310 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 95 CCACTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 944 GGGTCCT [GA]G^G[AT]CC[CT]
ScaI 152 AGTACT AGT^ACT
SmaI 71 CCCGGG CCC^GGG
SmlI 913 CTCAAG C^T[CT][GA]AG
StuI 233 AGGCCT AGG^CCT
StyI 422 CCAAGG C^C[AT][AT]GG
TaqI 308 TCGA T^CGA
TfiI 752 GATTC G^A[AT]TC
Tru9I 328 TTAA T^TAA
Tth111I 310 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 308 TCGA T^CGA
Van91I 95 CCACTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 71 CCCGGG C^CCGGG
XmaI 71 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 635 CGCG CG^CG
1024 CGCG
AcyI 8 GACGCC G[GA]^CG[CT]C
507 GACGTC
AspHI 664 GTGCAC G[AT]GC[AT]^C
768 GTGCTC
BsaHI 8 GACGCC G[GA]^CG[CT]C
507 GACGTC
BsgI 253 CTGCAC GTGCAG(16/14)
47 GTGCAG
BsiHKAI 664 GTGCAC G[AT]GC[AT]^C
768 GTGCTC
BsmAI 59 GAGAC GTCTC(1/5)
878 GAGAC
BsmFI 275 GGGAC GGGAC(10/14)
505 GGGAC
BstAPI 272 GCAGGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
953 GCATGAGTTGC
BstUI 635 CGCG CG^CG
1024 CGCG
DpnII 456 GATC ^GATC
988 GATC
DsaI 77 CCGTGG C^C[GA][CT]GG
773 CCACGG
EcoNI 912 CCTCAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1037 CCTGCCAGAGG
FauI 53 CCCGC CCCGC(4/6)
803 CCCGC
HaeII 817 AGCGCC [GA]GCGC^[CT]
1049 AGCGCT
HgaI 8 GACGC GACGC(5/10)
61 GACGC
Hin1I 8 GACGCC G[GA]^CG[CT]C
507 GACGTC
HphI 692 TCACC GGTGA(8/7)
800 TCACC
MaeII 508 ACGT A^CGT
541 ACGT
MboI 456 GATC ^GATC
988 GATC
MvnI 635 CGCG CG^CG
1024 CGCG
NdeII 456 GATC ^GATC
988 GATC
PstI 546 CTGCAG CTGCA^G
739 CTGCAG
Sau3AI 456 GATC ^GATC
988 GATC
SfaNI 67 GATGC GCATC(5/9)
500 GCATC
TspRI 180 CAGTG CA[GC]TG[ACGT][ACGT]^
980 CAGTG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
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AciI AfaI AluI AlwNI AvaI
AvaII BanI BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeIII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PleI PvuII RsaI
Sau96I ScrFI SfcI TseI Tsp45I