Restriction map for
pFN21AE5381
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglI BglII
BlpI Bpu1102I BsaAI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DrdI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MfeI MflI MluI
MroI MseI MspA1I MspI MunI
MvnI MwoI NaeI NarI NciI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tru9I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AhdI 734 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 684 AGCT AG^CT
Alw44I 469 GTGCAC G^TGCAC
AlwI 318 GATCC GGATC(4/5)
ApaLI 469 GTGCAC G^TGCAC
AspEI 734 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 523 CCTAGG C^CTAGG
BalI 757 TGGCCA TGG^CCA
BbvI 266 GCTGC GCAGC(8/12)
BclI 809 TGATCA T^GATCA
BlnI 523 CCTAGG C^CTAGG
BpmI 46 CTCCAG CTGGAG(16/14)
BsaBI 312 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 25 GGTCTC GGTCTC(1/5)
BsmFI 488 GTCCC GGGAC(10/14)
Bsp1407I 544 TGTACA T^GTACA
BsrGI 544 TGTACA T^GTACA
BsrI 453 CCAGT ACTGG(1/-1)
BssKI 755 CCTGG ^CC[ACGT]GG
BstNI 755 CCTGG CC^[AT]GG
CfoI 243 GCGC GCG^C
DraIII 597 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 329 CCATGG C^C[GA][CT]GG
EaeI 757 TGGCCA [CT]^GGCC[GA]
Eam1105I 734 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 10 GAATTC G^AATTC
EcoRII 755 CCTGG ^CC[AT]GG
FbaI 809 TGATCA T^GATCA
Fnu4HI 266 GCTGC GC^[ACGT]GC
HhaI 243 GCGC GCG^C
HinP1I 243 GCGC G^CGC
ItaI 266 GCTGC GC^[ACGT]GC
MamI 312 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 757 TGGCCA TGG^CCA
MscI 757 TGGCCA TGG^CCA
MvaI 755 CCTGG CC^[AT]GG
NcoI 329 CCATGG C^CATGG
NheI 165 GCTAGC G^CTAGC
NlaIV 487 GGTCCC GG[ACGT]^[ACGT]CC
NspI 397 GCATGT [GA]CATG^[CT]
PleI 133 GACTC GAGTC(4/5)
ScrFI 755 CCTGG CC^[ACGT]GG
SfaNI 892 GATGC GCATC(5/9)
SfcI 555 CTACAG C^T[GA][CT]AG
SspBI 544 TGTACA T^GTACA
TaqI 500 TCGA T^CGA
TseI 266 GCTGC G^C[AT]GC
TthHB8I 500 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 10 GAATTC [GA]^AATT[CT]
784 AAATTC
AfaI 156 GTAC GT^AC
545 GTAC
ApoI 10 GAATTC [GA]^AATT[CT]
784 AAATTC
AspHI 469 GTGCAC G[AT]GC[AT]^C
592 GTGCTC
AvaII 487 GGTCC G^G[AT]CC
733 GGACC
BbsI 861 GAAGAC GAAGAC(2/6)
198 GTCTTC
BmyI 469 GTGCAC G[AGT]GC[ACT]^C
592 GTGCTC
BpuAI 861 GAAGAC GAAGAC(2/6)
198 GTCTTC
BsaJI 329 CCATGG C^C[ACGT][ACGT]GG
523 CCTAGG
BsiHKAI 469 GTGCAC G[AT]GC[AT]^C
592 GTGCTC
BsiYI 478 CCCTTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
519 CCTTCCTAGGG
BslI 478 CCCTTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
519 CCTTCCTAGGG
BsmAI 26 GTCTC GTCTC(1/5)
192 GTCTC
Bsp1286I 469 GTGCAC G[AGT]GC[ACT]^C
592 GTGCTC
BsrDI 576 CATTGC GCAATG(2/0)
658 CATTGC
Cac8I 165 GCTAGC GC[ACGT]^[ACGT]GC
239 GCCAGC
Cfr13I 487 GGTCC G^G[ACGT]CC
733 GGACC
EcoT14I 329 CCATGG C^C[AT][AT]GG
523 CCTAGG
HaeIII 459 GGCC GG^CC
758 GGCC
RsaI 156 GTAC GT^AC
545 GTAC
Sau96I 487 GGTCC G^G[ACGT]CC
733 GGACC
StyI 329 CCATGG C^C[AT][AT]GG
523 CCTAGG
TfiI 34 GATTC G^A[AT]TC
89 GAATC
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
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BfaI BstF5I DdeI DpnII FokI
HinfI HphI MaeI MaeIII MboI
MboII MnlI MslI NdeII NlaIII
Sau3AI Tsp45I Tsp509I TspRI