Restriction map for
pFN21AE5351
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssKI BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MflI MluI MluNI
MroI MscI MvaI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PvuI
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StuI StyI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AclI 373 AACGTT AA^CGTT
Aor51HI 509 AGCGCT AGC^GCT
AseI 865 ATTAAT AT^TAAT
AsnI 865 ATTAAT AT^TAAT
Asp700I 5 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 9 GTCTTC GAAGAC(2/6)
BfaI 106 CTAG C^TAG
BpuAI 9 GTCTTC GAAGAC(2/6)
BseRI 84 CTCCTC GAGGAG(10/8)
BsgI 843 CTGCAC GTGCAG(16/14)
BsmAI 705 GTCTC GTCTC(1/5)
BsmI 286 GAATGC GAATGC(1/-1)
Bsp1407I 829 TGTACA T^GTACA
BsrGI 829 TGTACA T^GTACA
Cac8I 525 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 510 GCGC GCG^C
Eco47III 509 AGCGCT AGC^GCT
EcoRI 692 GAATTC G^AATTC
EcoRV 145 GATATC GAT^ATC
HaeII 509 AGCGCT [GA]GCGC^[CT]
HaeIII 524 GGCC GG^CC
HhaI 510 GCGC GCG^C
HinP1I 510 GCGC G^CGC
MaeI 106 CTAG C^TAG
MaeII 374 ACGT A^CGT
MaeIII 758 GTTAC ^GT[ACGT]AC
MfeI 591 CAATTG C^AATTG
MspA1I 140 CAGCTG C[AC]G^C[GT]G
MunI 591 CAATTG C^AATTG
MwoI 721 GCACCACCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 281 CATATG CA^TATG
NspI 882 ACATGC [GA]CATG^[CT]
PleI 453 GAGTC GAGTC(4/5)
PshBI 865 ATTAAT AT^TAAT
Psp1406I 373 AACGTT AA^CGTT
PstI 445 CTGCAG CTGCA^G
PvuII 140 CAGCTG CAG^CTG
SfaNI 643 GATGC GCATC(5/9)
SfcI 445 CTGCAG C^T[GA][CT]AG
SspBI 829 TGTACA T^GTACA
XmnI 5 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BbvI 172 GCAGC GCAGC(8/12)
512 GCTGC
BsaWI 51 ACCGGA [AT]^CCGG[AT]
837 ACCGGA
BsiYI 426 CCCAGTAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
887 CCTTGAAATGG
BslI 426 CCCAGTAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
887 CCTTGAAATGG
DpnII 205 GATC ^GATC
307 GATC
Fnu4HI 172 GCAGC GC^[ACGT]GC
512 GCTGC
HapII 52 CCGG C^CGG
838 CCGG
HpaII 52 CCGG C^CGG
838 CCGG
ItaI 172 GCAGC GC^[ACGT]GC
512 GCTGC
MboI 205 GATC ^GATC
307 GATC
MslI 281 CATATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
675 CACTCATGTG
MspI 52 CCGG C^CGG
838 CCGG
NdeII 205 GATC ^GATC
307 GATC
NlaIII 679 CATG CATG^
883 CATG
NlaIV 54 GGAGCC GG[ACGT]^[ACGT]CC
70 GGTTCC
Sau3AI 205 GATC ^GATC
307 GATC
ScaI 518 AGTACT AGT^ACT
699 AGTACT
SspI 112 AATATT AAT^ATT
269 AATATT
TaqI 422 TCGA T^CGA
900 TCGA
TfiI 325 GAATC G^A[AT]TC
897 GATTC
TseI 172 GCAGC G^C[AT]GC
512 GCTGC
TthHB8I 422 TCGA T^CGA
900 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AcsI AfaI AluI ApoI BsmFI
BsrI BstF5I DdeI FokI HinfI
HphI MboII MnlI MseI RsaI
Tru9I Tsp509I TspRI