Restriction map for
pFN21AE4204
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AhdI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BanII BbeI BbrPI BcgI
BciVI BfrI BglII BlnI BsaAI
BsaBI BseAI BseRI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I CfoI
ClaI CpoI DraII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRI EcoT22I FauI
FseI FspI HaeII HhaI HinP1I
HindIII KasI KpnI KspI MaeII
MamI MluI MroI MspA1I MvnI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 991 GGCGTC G[GA]^CG[CT]C
AgeI 2006 ACCGGT A^CCGGT
Alw44I 565 GTGCAC G^TGCAC
AocI 1078 CCTAAGG CC^T[ACGT]AGG
ApaLI 565 GTGCAC G^TGCAC
Asp700I 2016 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 717 TGGCCA TGG^CCA
BbvCI 1711 CCTCAGC CCTCAGC(-5/-2)
BclI 1341 TGATCA T^GATCA
BglI 1974 GCCCTATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1582 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1582 GCTGAGC GC^T[ACGT]AGC
BsaHI 991 GGCGTC G[GA]^CG[CT]C
BsaWI 2006 ACCGGT [AT]^CCGG[AT]
BspHI 2046 TCATGA T^CATGA
BsrFI 2006 ACCGGT [GA]^CCGG[CT]
BstEII 1238 GGTTACC G^GT[ACGT]ACC
BstPI 1238 GGTTACC G^GT[ACGT]ACC
BstXI 1984 CCAGGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1078 CCTAAGG CC^T[ACGT]AGG
CelII 1582 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2006 ACCGGT [GA]^CCGG[CT]
DraIII 568 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 717 TGGCCA [CT]^GGCC[GA]
Eco81I 1078 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1238 GGTTACC G^GT[ACGT]ACC
EcoRV 316 GATATC GAT^ATC
FbaI 1341 TGATCA T^GATCA
HgaI 992 GCGTC GACGC(5/10)
Hin1I 991 GGCGTC G[GA]^CG[CT]C
HincII 508 GTTAAC GT[CT]^[GA]AC
HindII 508 GTTAAC GT[CT]^[GA]AC
HpaI 508 GTTAAC GTT^AAC
MfeI 701 CAATTG C^AATTG
MluNI 717 TGGCCA TGG^CCA
MscI 717 TGGCCA TGG^CCA
MslI 1011 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 701 CAATTG C^AATTG
PflMI 1740 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2006 ACCGGT A^CCGGT
PstI 737 CTGCAG CTGCA^G
RcaI 2046 TCATGA T^CATGA
ScaI 1829 AGTACT AGT^ACT
Van91I 1740 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2016 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 595 GTCTAC GT^[AC][GT]AC
1387 GTAGAC
AciI 1597 CCGC CCGC(-3/-1)
876 GCGG
AlwNI 740 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1286 CAGATGCTG
AvaII 307 GGACC G^G[AT]CC
1086 GGACC
BamHI 188 GGATCC G^GATCC
1887 GGATCC
BanI 1002 GGCACC G^G[CT][GA]CC
1946 GGCACC
BbsI 1423 GAAGAC GAAGAC(2/6)
1512 GTCTTC
BcnI 45 CCCGG CC^[GC]GG
1171 CCGGG
BpmI 25 CTCCAG CTGGAG(16/14)
1675 CTGGAG
BpuAI 1423 GAAGAC GAAGAC(2/6)
1512 GTCTTC
BsaI 1678 GAGACC GGTCTC(1/5)
2009 GGTCTC
BsmAI 1678 GAGAC GTCTC(1/5)
2010 GTCTC
BsmI 267 GAATGC GAATGC(1/-1)
361 GCATTC
BsrDI 1124 CATTGC GCAATG(2/0)
1970 CATTGC
DraI 1410 TTTAAA TTT^AAA
1687 TTTAAA
NciI 45 CCCGG CC^[GC]GG
1171 CCGGG
NspI 109 ACATGC [GA]CATG^[CT]
541 GCATGT
SexAI 1458 ACCTGGT A^CC[AT]GGT
1681 ACCAGGT
StuI 607 AGGCCT AGG^CCT
1091 AGGCCT
Tsp45I 578 GTGAC ^GT[GC]AC
1550 GTGAC
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AfaI AluI AlwI AspHI BbvI
BfaI BmyI BsaJI BsgI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NheI NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XcmI XhoII