Restriction map for
pFN21AE5817
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbvCI BcgI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BspDI BspEI BspLU11I BsrBI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoT22I
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Alw44I 601 GTGCAC G^TGCAC
Aor51HI 1157 AGCGCT AGC^GCT
ApaI 2057 GGGCCC GGGCC^C
ApaLI 601 GTGCAC G^TGCAC
BamHI 203 GGATCC G^GATCC
BfaI 1423 CTAG C^TAG
BsaBI 39 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1651 GAGACG CGTCTC(1/5)
BsmI 476 GCATTC GAATGC(1/-1)
BspHI 2304 TCATGA T^CATGA
BsrDI 398 GCAATG GCAATG(2/0)
BsrFI 98 ACCGGC [GA]^CCGG[CT]
BsrI 2438 CCAGT ACTGG(1/-1)
BstAPI 171 GCACACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 98 ACCGGC [GA]^CCGG[CT]
DraI 2314 TTTAAA TTT^AAA
Eco47III 1157 AGCGCT AGC^GCT
EcoRI 586 GAATTC G^AATTC
HincII 715 GTCAAC GT[CT]^[GA]AC
HindII 715 GTCAAC GT[CT]^[GA]AC
KspI 48 CCGCGG CCGC^GG
MaeI 1423 CTAG C^TAG
MaeIII 2441 GTGAC ^GT[ACGT]AC
MamI 39 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 677 CATATG CA^TATG
NruI 1034 TCGCGA TCG^CGA
PleI 2364 GAGTC GAGTC(4/5)
PpuMI 833 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 2006 CTGCAG CTGCA^G
RcaI 2304 TCATGA T^CATGA
SacII 48 CCGCGG CCGC^GG
SmaI 830 CCCGGG CCC^GGG
SmlI 656 CTCAAG C^T[CT][GA]AG
StuI 2387 AGGCCT AGG^CCT
TaqI 1194 TCGA T^CGA
Tsp45I 2441 GTGAC ^GT[GC]AC
TthHB8I 1194 TCGA T^CGA
XmaCI 830 CCCGGG C^CCGGG
XmaI 830 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwNI 968 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
1642 CAGTTCCTG
BciVI 469 GGATAC GTATCC(6/5)
1709 GGATAC
BglII 1983 AGATCT A^GATCT
2493 AGATCT
Bsp1407I 1760 TGTACA T^GTACA
2372 TGTACA
BspMI 104 ACCTGC ACCTGC(4/8)
525 GCAGGT
BsrGI 1760 TGTACA T^GTACA
2372 TGTACA
BstXI 1465 CCAAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2383 CCAAAGGCCTGG
FauI 146 CCCGC CCCGC(4/6)
578 CCCGC
NcoI 440 CCATGG C^CATGG
2075 CCATGG
NspI 1746 GCATGT [GA]CATG^[CT]
2291 ACATGC
PflMI 186 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1088 CCAAGGCATGG
SacI 1099 GAGCTC GAGCT^C
1504 GAGCTC
SexAI 188 ACCTGGT A^CC[AT]GGT
1172 ACCTGGT
SfcI 1944 CTACAG C^T[GA][CT]AG
2006 CTGCAG
SspBI 1760 TGTACA T^GTACA
2372 TGTACA
Van91I 186 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1088 CCAAGGCATGG
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BclI BcnI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoRV EcoT14I FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp509I TspRI XcmI XhoII XmnI