Restriction map for
pFN21AE6180
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaWI BseAI BseRI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeI MaeII MboII MfeI MflI
MluI MroI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuII RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 62 AAATTT [GA]^AATT[CT]
AcyI 316 GACGCC G[GA]^CG[CT]C
ApoI 62 AAATTT [GA]^AATT[CT]
Asp700I 59 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 240 TGGCCA TGG^CCA
BclI 351 TGATCA T^GATCA
BpmI 111 CTCCAG CTGGAG(16/14)
BsaBI 157 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 316 GACGCC G[GA]^CG[CT]C
BsaI 12 GGTCTC GGTCTC(1/5)
BsgI 35 GTGCAG GTGCAG(16/14)
BsiEI 311 CGATCG CG[GA][CT]^CG
BsmAI 13 GTCTC GTCTC(1/5)
BsmI 135 GAATGC GAATGC(1/-1)
BspHI 335 TCATGA T^CATGA
BssKI 243 CCAGG ^CC[ACGT]GG
BstNI 243 CCAGG CC^[AT]GG
DdeI 17 CTGAG C^T[ACGT]AG
DraII 282 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 240 TGGCCA [CT]^GGCC[GA]
EcoO109I 282 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 243 CCAGG ^CC[AT]GG
FbaI 351 TGATCA T^GATCA
HgaI 316 GACGC GACGC(5/10)
Hin1I 316 GACGCC G[GA]^CG[CT]C
HphI 29 TCACC GGTGA(8/7)
MaeIII 107 GTGAC ^GT[ACGT]AC
MamI 157 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 240 TGGCCA TGG^CCA
MscI 240 TGGCCA TGG^CCA
MseI 146 TTAA T^TAA
MvaI 243 CCAGG CC^[AT]GG
NlaIV 220 GGGGCC GG[ACGT]^[ACGT]CC
PvuI 311 CGATCG CGAT^CG
RcaI 335 TCATGA T^CATGA
ScrFI 243 CCAGG CC^[ACGT]GG
SspI 370 AATATT AAT^ATT
TaqI 314 TCGA T^CGA
TfiI 303 GATTC G^A[AT]TC
Tru9I 146 TTAA T^TAA
Tsp45I 107 GTGAC ^GT[GC]AC
Tsp509I 63 AATT ^AATT
TspRI 140 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 314 TCGA T^CGA
XcmI 69 CCAGCAGTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 59 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AciI 10 GCGG CCGC(-3/-1)
193 GCGG
BbvI 21 GCAGC GCAGC(8/12)
210 GCTGC
BsiYI 183 CCCTGATAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
237 CCATGGCCAGG
BslI 183 CCCTGATAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
237 CCATGGCCAGG
BstF5I 197 CATCC GGATG(2/0)
174 GGATG
CfoI 213 GCGC GCG^C
231 GCGC
FokI 197 CATCC GGATG(9/13)
174 GGATG
HhaI 213 GCGC GCG^C
231 GCGC
HinP1I 213 GCGC G^CGC
231 GCGC
MslI 98 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
197 CATCCGAGTG
MwoI 233 GCTACCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
310 GCGATCGACGC
TseI 21 GCAGC G^C[AT]GC
210 GCTGC
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
BsaJI Cfr13I DpnII DsaI EcoT14I
Fnu4HI HaeIII HinfI ItaI MboI
MnlI NcoI NdeII NlaIII PleI
Sau3AI Sau96I SfaNI StyI