Restriction map for
pFN21AE1163
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AvrII BalI BanI
BbeI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoT22I FbaI
FseI HaeII HapII HgaI HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 1144 GACGTC GACGT^C
AcyI 1144 GACGTC G[GA]^CG[CT]C
Asp700I 784 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 15 GAGCTC G[AT]GC[AT]^C
AspI 875 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1220 GGACC G^G[AT]CC
AviII 1241 TGCGCA TGC^GCA
BamHI 1658 GGATCC G^GATCC
BbrPI 9 CACGTG CAC^GTG
BbsI 1374 GAAGAC GAAGAC(2/6)
BbvCI 1513 GCTGAGG CCTCAGC(-5/-2)
BciVI 969 GGATAC GTATCC(6/5)
BpmI 667 CTGGAG CTGGAG(16/14)
BpuAI 1374 GAAGAC GAAGAC(2/6)
BsaAI 9 CACGTG [CT]AC^GT[GA]
BsaHI 1144 GACGTC G[GA]^CG[CT]C
BsaI 400 GGTCTC GGTCTC(1/5)
BseRI 844 GAGGAG GAGGAG(10/8)
BsiHKAI 15 GAGCTC G[AT]GC[AT]^C
BspLU11I 955 ACATGT A^CATGT
BspMI 973 ACCTGC ACCTGC(4/8)
BsrDI 275 CATTGC GCAATG(2/0)
CfoI 1242 GCGC GCG^C
EcoRI 820 GAATTC G^AATTC
EcoRV 1129 GATATC GAT^ATC
FauI 54 GCGGG CCCGC(4/6)
FspI 1241 TGCGCA TGC^GCA
HhaI 1242 GCGC GCG^C
Hin1I 1144 GACGTC G[GA]^CG[CT]C
HinP1I 1242 GCGC G^CGC
PaeR7I 797 CTCGAG C^TCGAG
PflFI 875 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1485 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 9 CACGTG CAC^GTG
PmlI 9 CACGTG CAC^GTG
PpuMI 1219 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 40 CAGCTG CAG^CTG
SacI 15 GAGCTC GAGCT^C
Tth111I 875 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1485 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 797 CTCGAG C^TCGAG
XmnI 784 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 8 ACACGT A^C[GA][CT]GT
955 ACATGT
ApaI 492 GGGCCC GGGCC^C
806 GGGCCC
AvaI 87 CTCGGG C^[CT]CG[GA]G
797 CTCGAG
BfaI 739 CTAG C^TAG
1669 CTAG
BsmFI 511 GGGAC GGGAC(10/14)
1219 GGGAC
BsoBI 87 CTCGGG C^[CT]CG[GA]G
797 CTCGAG
DraIII 1067 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1612 CACAATGTG
DsaI 1307 CCATGG C^C[GA][CT]GG
1431 CCATGG
MaeI 739 CTAG C^TAG
1669 CTAG
MaeII 10 ACGT A^CGT
1145 ACGT
MslI 916 CACACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1109 CACCTCAGTG
MspA1I 40 CAGCTG C[AC]G^C[GT]G
67 CAGCGG
NcoI 1307 CCATGG C^CATGG
1431 CCATGG
NspI 645 ACATGC [GA]CATG^[CT]
955 ACATGT
PstI 64 CTGCAG CTGCA^G
358 CTGCAG
SmlI 335 CTCAAG C^T[CT][GA]AG
797 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BanII BbvI BmyI
BsaBI BsaJI BsgI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MamI MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII