##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     Cac8I       
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HapII       HgaI        Hin1I       
HincII      HindII      HpaI        HpaII       KasI        
KpnI        KspI        MaeI        MaeII       MamI        
MfeI        MluI        MroI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
TaqI        Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AccI          721  GTAGAC               GT^[AC][GT]AC
AcsI          652  AAATTC               [GA]^AATT[CT]
ApoI          652  AAATTC               [GA]^AATT[CT]
BalI          410  TGGCCA               TGG^CCA
BanI          117  GGTGCC               G^G[CT][GA]CC
BseRI         372  CTCCTC               GAGGAG(10/8)
BsmAI         561  GTCTC                GTCTC(1/5)
Bsp1407I      254  TGTACA               T^GTACA
BsrGI         254  TGTACA               T^GTACA
BstXI         263  CCACTCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         740  AGATCC               [GA]^GATC[CT]
CfoI          933  GCGC                 GCG^C
DraIII        731  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          410  TGGCCA               [CT]^GGCC[GA]
FauI          466  GCGGG                CCCGC(4/6)
HhaI          933  GCGC                 GCG^C
HinP1I        933  GCGC                 G^CGC
HindIII      1049  AAGCTT               A^AGCTT
MflI          740  AGATCC               [GA]^GATC[CT]
MluNI         410  TGGCCA               TGG^CCA
MscI          410  TGGCCA               TGG^CCA
NcoI          503  CCATGG               C^CATGG
NspI          251  GCATGT               [GA]CATG^[CT]
SexAI         278  ACCTGGT              A^CC[AT]GGT
SfaNI         877  GCATC                GCATC(5/9)
SfcI          597  CTACAG               C^T[GA][CT]AG
SspBI         254  TGTACA               T^GTACA
TfiI          608  GATTC                G^A[AT]TC
Tsp45I        673  GTCAC                ^GT[GC]AC
XhoII         740  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AocI           62  CCTTAGG              CC^T[ACGT]AGG
              565  CCTGAGG             
AvaII         941  GGTCC                G^G[AT]CC
              963  GGACC               
BpmI          220  CTCCAG               CTGGAG(16/14)
              144  CTGGAG              
BspMI         929  ACCTGC               ACCTGC(4/8)
              851  GCAGGT              
Bsu36I         62  CCTTAGG              CC^T[ACGT]AGG
              565  CCTGAGG             
DsaI          377  CCACGG               C^C[GA][CT]GG
              503  CCATGG              
Eco81I         62  CCTTAGG              CC^T[ACGT]AGG
              565  CCTGAGG             
EcoT14I       174  CCAAGG               C^C[AT][AT]GG
              503  CCATGG              
MaeIII        533  GTAAC                ^GT[ACGT]AC
              673  GTCAC               
PflMI         174  CCAAGGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              390  CCAGACCCTGG         
PleI           41  GACTC                GAGTC(4/5)
              447  GACTC               
SacI          190  GAGCTC               GAGCT^C
              837  GAGCTC              
StyI          174  CCAAGG               C^C[AT][AT]GG
              503  CCATGG              
Van91I        174  CCAAGGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              390  CCAGACCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       BanII       BbvI        BmyI        BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DpnII       EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
Ksp632I     MboI        MboII       MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       Tru9I       TseI        Tsp509I     TspRI