Restriction map for
pFN21AE5990
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BspMI BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeI MamI MfeI MflI
MluI MroI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TaqI Tth111I TthHB8I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AfaI 719 GTAC GT^AC
AflIII 773 ACATGT A^C[GA][CT]GT
AlwI 694 GGATC GGATC(4/5)
BanII 46 GGGCTC G[GA]GC[CT]^C
BbsI 739 GTCTTC GAAGAC(2/6)
BciVI 570 GGATAC GTATCC(6/5)
BmyI 46 GGGCTC G[AGT]GC[ACT]^C
BpuAI 739 GTCTTC GAAGAC(2/6)
BsgI 388 CTGCAC GTGCAG(16/14)
BsmAI 213 GTCTC GTCTC(1/5)
BsmI 462 GCATTC GAATGC(1/-1)
Bsp1286I 46 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 718 TGTACA T^GTACA
BspLU11I 773 ACATGT A^CATGT
BsrGI 718 TGTACA T^GTACA
BssSI 430 CTCGTG CACGAG(-5/-1)
BstF5I 3 GGATG GGATG(2/0)
Cac8I 380 GCAAGC GC[ACGT]^[ACGT]GC
DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoNI 356 CCTTTGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
FokI 3 GGATG GGATG(9/13)
HphI 159 TCACC GGTGA(8/7)
MaeII 493 ACGT A^CGT
MwoI 220 GCTTCTTTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 650 CATATG CA^TATG
NspI 773 ACATGT [GA]CATG^[CT]
PflMI 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 471 GAGTC GAGTC(4/5)
RsaI 719 GTAC GT^AC
SfcI 67 CTACAG C^T[GA][CT]AG
SspBI 718 TGTACA T^GTACA
TspRI 321 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 312 CCACCTTTTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 18 GTCTAC GT^[AC][GT]AC
520 GTAGAC
AcsI 31 GAATTT [GA]^AATT[CT]
918 AAATTT
ApoI 31 GAATTT [GA]^AATT[CT]
918 AAATTT
AvaII 153 GGACC G^G[AT]CC
508 GGTCC
BalI 347 TGGCCA TGG^CCA
778 TGGCCA
BbvI 788 GCAGC GCAGC(8/12)
287 GCTGC
BsaJI 59 CCTGGG C^C[ACGT][ACGT]GG
413 CCCAGG
BsmFI 410 GTCCC GGGAC(10/14)
509 GTCCC
BsrDI 95 CATTGC GCAATG(2/0)
931 GCAATG
BsrI 322 ACTGG ACTGG(1/-1)
555 CCAGT
EaeI 347 TGGCCA [CT]^GGCC[GA]
778 TGGCCA
EcoT22I 460 ATGCAT ATGCA^T
759 ATGCAT
Fnu4HI 287 GCTGC GC^[ACGT]GC
788 GCAGC
ItaI 287 GCTGC GC^[ACGT]GC
788 GCAGC
MluNI 347 TGGCCA TGG^CCA
778 TGGCCA
MscI 347 TGGCCA TGG^CCA
778 TGGCCA
MseI 644 TTAA T^TAA
923 TTAA
NlaIV 153 GGACCC GG[ACGT]^[ACGT]CC
508 GGTCCC
NsiI 460 ATGCAT ATGCA^T
759 ATGCAT
SfaNI 678 GCATC GCATC(5/9)
761 GCATC
TfiI 807 GATTC G^A[AT]TC
870 GAATC
Tru9I 644 TTAA T^TAA
923 TTAA
TseI 287 GCTGC G^C[AT]GC
788 GCAGC
Tsp45I 263 GTCAC ^GT[GC]AC
536 GTGAC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
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AluI BsiYI BslI BssKI BstNI
Cfr13I DdeI DpnII EcoRII HaeIII
HinfI MaeIII MboI MboII MnlI
MslI MvaI NdeII NlaIII Sau3AI
Sau96I ScrFI Tsp509I