Restriction map for
pFN21AE0188
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII AccI AccII AcyI AflII
AgeI AhdI Alw44I AlwI AocI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspHI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI Ksp632I KspI MamI
MfeI MluI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 426 GGTACC G^GTACC
AccIII 342 TCCGGA T^CCGGA
AciI 24 GCGG CCGC(-3/-1)
AclI 316 AACGTT AA^CGTT
AflIII 181 ACATGT A^C[GA][CT]GT
AlwNI 392 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 5 AGCGCT AGC^GCT
Asp718I 426 GGTACC G^GTACC
AvaII 789 GGTCC G^G[AT]CC
BalI 817 TGGCCA TGG^CCA
BanI 426 GGTACC G^G[CT][GA]CC
BbvI 21 GCAGC GCAGC(8/12)
BciVI 353 GGATAC GTATCC(6/5)
BfaI 232 CTAG C^TAG
BglII 561 AGATCT A^GATCT
BsaJI 497 CCCTGG C^C[ACGT][ACGT]GG
BsaWI 342 TCCGGA [AT]^CCGG[AT]
BseAI 342 TCCGGA T^CCGGA
BsmAI 367 GAGAC GTCTC(1/5)
BsmI 462 GCATTC GAATGC(1/-1)
BspEI 342 TCCGGA T^CCGGA
BspLU11I 181 ACATGT A^CATGT
BsrDI 763 CATTGC GCAATG(2/0)
BssSI 709 CTCGTG CACGAG(-5/-1)
BstAPI 130 GCATTAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 561 AGATCT [GA]^GATC[CT]
CfoI 6 GCGC GCG^C
Cfr13I 789 GGTCC G^G[ACGT]CC
DdeI 567 CTTAG C^T[ACGT]AG
EaeI 817 TGGCCA [CT]^GGCC[GA]
Eco47III 5 AGCGCT AGC^GCT
EcoNI 71 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 840 GAATTC G^AATTC
Fnu4HI 21 GCAGC GC^[ACGT]GC
HaeII 5 AGCGCT [GA]GCGC^[CT]
HaeIII 818 GGCC GG^CC
HapII 343 CCGG C^CGG
HhaI 6 GCGC GCG^C
HinP1I 6 GCGC G^CGC
HpaII 343 CCGG C^CGG
HphI 54 GGTGA GGTGA(8/7)
ItaI 21 GCAGC GC^[ACGT]GC
KpnI 426 GGTACC GGTAC^C
MaeI 232 CTAG C^TAG
MaeII 317 ACGT A^CGT
MflI 561 AGATCT [GA]^GATC[CT]
MluNI 817 TGGCCA TGG^CCA
MroI 342 TCCGGA T^CCGGA
MscI 817 TGGCCA TGG^CCA
MspA1I 22 CAGCGG C[AC]G^C[GT]G
MspI 343 CCGG C^CGG
NdeI 614 CATATG CA^TATG
NspI 181 ACATGT [GA]CATG^[CT]
Psp1406I 316 AACGTT AA^CGTT
Sau96I 789 GGTCC G^G[ACGT]CC
SfcI 470 CTGTAG C^T[GA][CT]AG
SspI 963 AATATT AAT^ATT
TaqI 228 TCGA T^CGA
TfiI 523 GATTC G^A[AT]TC
TseI 21 GCAGC G^C[AT]GC
TthHB8I 228 TCGA T^CGA
XhoII 561 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 427 GTAC GT^AC
502 GTAC
AluI 255 AGCT AG^CT
339 AGCT
BanII 12 GGGCTC G[GA]GC[CT]^C
224 GGGCTC
BmyI 12 GGGCTC G[AGT]GC[ACT]^C
224 GGGCTC
BseRI 61 GAGGAG GAGGAG(10/8)
322 GAGGAG
BsgI 19 GTGCAG GTGCAG(16/14)
545 GTGCAG
BsiYI 17 CCGTGCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
71 CCTCCAAAAGG
BslI 17 CCGTGCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
71 CCTCCAAAAGG
Bsp1286I 12 GGGCTC G[AGT]GC[ACT]^C
224 GGGCTC
BstXI 214 CCATACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
798 CCATCATCTTGG
Cac8I 458 GCTGGC GC[ACGT]^[ACGT]GC
815 GCTGGC
MboII 724 GAAGA GAAGA(8/7)
648 TCTTC
MslI 514 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
763 CATTGCAGTG
PleI 883 GACTC GAGTC(4/5)
950 GACTC
RsaI 427 GTAC GT^AC
502 GTAC
SfaNI 136 GATGC GCATC(5/9)
84 GCATC
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AcsI ApoI BsrI BssKI BstF5I
BstNI DpnII DraI EcoRII FokI
HinfI MaeIII MboI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI ScrFI Tru9I Tsp45I Tsp509I
TspRI