Restriction map for
pFN21AE3459
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AviII BfrI
BsaBI BsiWI BsmBI BspDI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DrdI Eam1105I Eco47III EcoRV
EcoT22I FseI FspI HpaI KpnI
KspI MamI MluI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SpeI SphI
Sse8387I StuI SwaI XbaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 1160 TCCGGA T^CCGGA
AcyI 2003 GGCGCC G[GA]^CG[CT]C
Alw44I 2167 GTGCAC G^TGCAC
ApaLI 2167 GTGCAC G^TGCAC
Asp700I 273 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 974 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2183 GGATCC G^GATCC
BbeI 2003 GGCGCC GGCGC^C
BbrPI 3122 CACGTG CAC^GTG
BbvCI 3090 CCTCAGC CCTCAGC(-5/-2)
BcgI 231 GCAGTGGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2801 AGATCT A^GATCT
BlpI 3316 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3316 GCTGAGC GC^T[ACGT]AGC
BsaHI 2003 GGCGCC G[GA]^CG[CT]C
BseAI 1160 TCCGGA T^CCGGA
BsiEI 1462 CGGCCG CG[GA][CT]^CG
Bsp1407I 146 TGTACA T^GTACA
BspEI 1160 TCCGGA T^CCGGA
BsrBI 2782 GAGCGG CCGCTC(-3/-3)
BsrGI 146 TGTACA T^GTACA
BssSI 262 CTCGTG CACGAG(-5/-1)
BstAPI 1469 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1183 GGTTACC G^GT[ACGT]ACC
BstPI 1183 GGTTACC G^GT[ACGT]ACC
CelII 3316 GCTGAGC GC^T[ACGT]AGC
DraI 2872 TTTAAA TTT^AAA
DraIII 1750 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1462 CGGCCG C^GGCCG
EclXI 1462 CGGCCG C^GGCCG
Eco52I 1462 CGGCCG C^GGCCG
EcoNI 899 CCTGTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1183 GGTTACC G^GT[ACGT]ACC
EcoRI 277 GAATTC G^AATTC
FauI 3923 CCCGC CCCGC(4/6)
HgaI 13 GACGC GACGC(5/10)
Hin1I 2003 GGCGCC G[GA]^CG[CT]C
HincII 3466 GTCAAC GT[CT]^[GA]AC
HindII 3466 GTCAAC GT[CT]^[GA]AC
KasI 2003 GGCGCC G^GCGCC
MroI 1160 TCCGGA T^CCGGA
NaeI 737 GCCGGC GCC^GGC
NarI 2003 GGCGCC GG^CGCC
NdeI 2275 CATATG CA^TATG
NgoMIV 737 GCCGGC G^CCGGC
PflFI 974 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3122 CACGTG CAC^GTG
PmlI 3122 CACGTG CAC^GTG
PshAI 321 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3319 GAGCTC GAGCT^C
SapI 1435 GAAGAGC GCTCTTC(1/4)
SfoI 2003 GGCGCC GGC^GCC
SnaBI 3938 TACGTA TAC^GTA
SspBI 146 TGTACA T^GTACA
SspI 2363 AATATT AAT^ATT
Tth111I 974 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 273 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 277 GAATTC [GA]^AATT[CT]
1624 GAATTT
AocI 712 CCTCAGG CC^T[ACGT]AGG
2188 CCTGAGG
ApoI 277 GAATTC [GA]^AATT[CT]
1624 GAATTT
AvrII 1779 CCTAGG C^CTAGG
3634 CCTAGG
BciVI 3157 GGATAC GTATCC(6/5)
2645 GTATCC
BclI 1091 TGATCA T^GATCA
1292 TGATCA
BglI 1630 GCCCATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3832 GCCATGGGGGC
BlnI 1779 CCTAGG C^CTAGG
3634 CCTAGG
BsaAI 3122 CACGTG [CT]AC^GT[GA]
3938 TACGTA
BsaWI 1160 TCCGGA [AT]^CCGG[AT]
3602 ACCGGA
BsmI 1642 GAATGC GAATGC(1/-1)
1663 GAATGC
BsrDI 293 CATTGC GCAATG(2/0)
1694 GCAATG
Bsu36I 712 CCTCAGG CC^T[ACGT]AGG
2188 CCTGAGG
Eco81I 712 CCTCAGG CC^T[ACGT]AGG
2188 CCTGAGG
FbaI 1091 TGATCA T^GATCA
1292 TGATCA
HindIII 460 AAGCTT A^AGCTT
2131 AAGCTT
MfeI 479 CAATTG C^AATTG
2810 CAATTG
MunI 479 CAATTG C^AATTG
2810 CAATTG
PaeR7I 1032 CTCGAG C^TCGAG
1619 CTCGAG
PpuMI 319 GGGACCC [GA]G^G[AT]CC[CT]
585 GGGTCCT
XhoI 1032 CTCGAG C^TCGAG
1619 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI