##################################
# NO CUT ENZYMES (  200 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BcnI        BfrI        BglI        BglII       BlnI        
BpmI        BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsiYI       BslI        BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FokI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeII       
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsaI        RsrII       SacI        SacII       SalI        
Sau96I      SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Asp700I        25  GAATTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         820  GTGCTC               G[AT]GC[AT]^C
BbvI          340  GCTGC                GCAGC(8/12)
BfaI          337  CTAG                 C^TAG
BlpI          685  GCTCAGC              GC^T[ACGT]AGC
BmyI          820  GTGCTC               G[AGT]GC[ACT]^C
Bpu1102I      685  GCTCAGC              GC^T[ACGT]AGC
BsaJI         254  CCAGGG               C^C[ACGT][ACGT]GG
BsiHKAI       820  GTGCTC               G[AT]GC[AT]^C
BsmAI         736  GTCTC                GTCTC(1/5)
BsmFI          39  GGGAC                GGGAC(10/14)
BsmI          224  GCATTC               GAATGC(1/-1)
Bsp1286I      820  GTGCTC               G[AGT]GC[ACT]^C
BspHI         398  TCATGA               T^CATGA
BspMI         712  ACCTGC               ACCTGC(4/8)
BsrDI         129  CATTGC               GCAATG(2/0)
BssKI         254  CCAGG                ^CC[ACGT]GG
BstNI         254  CCAGG                CC^[AT]GG
Cac8I         220  GCTTGC               GC[ACGT]^[ACGT]GC
CelII         685  GCTCAGC              GC^T[ACGT]AGC
EarI          717  CTCTTC               CTCTTC(1/4)
EcoRII        254  CCAGG                ^CC[AT]GG
Fnu4HI        340  GCTGC                GC^[ACGT]GC
HphI          242  TCACC                GGTGA(8/7)
ItaI          340  GCTGC                GC^[ACGT]GC
Ksp632I       717  CTCTTC               CTCTTC(1/4)
MaeI          337  CTAG                 C^TAG
MvaI          254  CCAGG                CC^[AT]GG
MwoI          892  GCCTTCAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI          162  GACTC                GAGTC(4/5)
RcaI          398  TCATGA               T^CATGA
SapI          716  GCTCTTC              GCTCTTC(1/4)
ScrFI         254  CCAGG                CC^[ACGT]GG
SmlI          157  CTCAAG               C^T[CT][GA]AG
TseI          340  GCTGC                G^C[AT]GC
TspRI         470  CACTG                CA[GC]TG[ACGT][ACGT]^
XmnI           25  GAATTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

BclI          455  TGATCA               T^GATCA
              516  TGATCA              
BsrI          445  ACTGG                ACTGG(1/-1)
              576  ACTGG               
FbaI          455  TGATCA               T^GATCA
              516  TGATCA              
Tsp45I        530  GTGAC                ^GT[GC]AC
              619  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AcsI        AluI        ApoI        DdeI        DpnII       
HinfI       MaeIII      MboI        MboII       MnlI        
MseI        NdeII       NlaIII      Sau3AI      TfiI        
Tru9I       Tsp509I