Restriction map for
pFN21AE6009
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII BalI BanI BanII
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MluI
MluNI MroI MscI MspA1I MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AciI 357 CCGC CCGC(-3/-1)
AcyI 162 GACGCC G[GA]^CG[CT]C
AvrII 36 CCTAGG C^CTAGG
BamHI 147 GGATCC G^GATCC
BbsI 892 GTCTTC GAAGAC(2/6)
BciVI 802 GTATCC GTATCC(6/5)
BglII 551 AGATCT A^GATCT
BlnI 36 CCTAGG C^CTAGG
BpuAI 892 GTCTTC GAAGAC(2/6)
BsaHI 162 GACGCC G[GA]^CG[CT]C
BsiYI 306 CCTTTTACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 306 CCTTTTACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 480 GGGAC GGGAC(10/14)
BspMI 879 GCAGGT ACCTGC(4/8)
BstAPI 407 GCAAAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 336 CCTGG CC^[AT]GG
EarI 678 CTCTTC CTCTTC(1/4)
EcoRII 336 CCTGG ^CC[AT]GG
EcoT14I 36 CCTAGG C^C[AT][AT]GG
HgaI 162 GACGC GACGC(5/10)
Hin1I 162 GACGCC G[GA]^CG[CT]C
Ksp632I 678 CTCTTC CTCTTC(1/4)
MaeII 362 ACGT A^CGT
MfeI 833 CAATTG C^AATTG
MslI 213 CACAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 833 CAATTG C^AATTG
MvaI 336 CCTGG CC^[AT]GG
NdeI 746 CATATG CA^TATG
NlaIV 147 GGATCC GG[ACGT]^[ACGT]CC
PleI 482 GACTC GAGTC(4/5)
PstI 547 CTGCAG CTGCA^G
SfcI 547 CTGCAG C^T[GA][CT]AG
SspI 207 AATATT AAT^ATT
StyI 36 CCTAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcsI 673 AAATTC [GA]^AATT[CT]
915 GAATTT
AfaI 427 GTAC GT^AC
716 GTAC
AlwI 148 GATCC GGATC(4/5)
147 GGATC
ApoI 673 AAATTC [GA]^AATT[CT]
915 GAATTT
BbvI 404 GCAGC GCAGC(8/12)
340 GCTGC
BcnI 152 CCCGG CC^[GC]GG
313 CCGGG
BsaJI 36 CCTAGG C^C[ACGT][ACGT]GG
151 CCCCGG
BsgI 558 CTGCAC GTGCAG(16/14)
683 CTGCAC
BsmAI 257 GAGAC GTCTC(1/5)
736 GTCTC
BsrI 445 ACTGG ACTGG(1/-1)
624 ACTGG
BstF5I 132 CATCC GGATG(2/0)
900 GGATG
BstYI 147 GGATCC [GA]^GATC[CT]
551 AGATCT
DpnII 148 GATC ^GATC
552 GATC
Fnu4HI 340 GCTGC GC^[ACGT]GC
404 GCAGC
FokI 132 CATCC GGATG(9/13)
900 GGATG
HapII 153 CCGG C^CGG
313 CCGG
HpaII 153 CCGG C^CGG
313 CCGG
ItaI 340 GCTGC GC^[ACGT]GC
404 GCAGC
MboI 148 GATC ^GATC
552 GATC
MflI 147 GGATCC [GA]^GATC[CT]
551 AGATCT
MspI 153 CCGG C^CGG
313 CCGG
MwoI 156 GCTCAAGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
407 GCAAAGTCTGC
NciI 152 CCCGG CC^[GC]GG
313 CCGGG
NdeII 148 GATC ^GATC
552 GATC
RsaI 427 GTAC GT^AC
716 GTAC
Sau3AI 148 GATC ^GATC
552 GATC
TseI 340 GCTGC G^C[AT]GC
404 GCAGC
XhoII 147 GGATCC [GA]^GATC[CT]
551 AGATCT
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
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AluI BfaI BssKI DdeI HinfI
HphI MaeI MaeIII MboII MnlI
MseI NlaIII ScrFI SmlI TfiI
Tru9I Tsp45I Tsp509I TspRI