Restriction map for
pFN21AE0835
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BamHI
BanI BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeII MaeIII
MamI MflI MluI MroI MspI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI StuI SwaI TaqI
Tsp45I TspRI Tth111I TthHB8I Van91I
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AfaI 792 GTAC GT^AC
AlwI 104 GGATC GGATC(4/5)
Asp700I 69 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 657 GGGCTC G[GA]GC[CT]^C
BbsI 121 GTCTTC GAAGAC(2/6)
BbvCI 363 GCTGAGG CCTCAGC(-5/-2)
BbvI 870 GCTGC GCAGC(8/12)
BmyI 657 GGGCTC G[AGT]GC[ACT]^C
BpmI 669 CTCCAG CTGGAG(16/14)
BpuAI 121 GTCTTC GAAGAC(2/6)
BsmFI 207 GGGAC GGGAC(10/14)
Bsp1286I 657 GGGCTC G[AGT]GC[ACT]^C
BssKI 290 CCTGG ^CC[ACGT]GG
BssSI 831 CTCGTG CACGAG(-5/-1)
BstNI 290 CCTGG CC^[AT]GG
DpnII 105 GATC ^GATC
DraI 540 TTTAAA TTT^AAA
EcoRI 46 GAATTC G^AATTC
EcoRII 290 CCTGG ^CC[AT]GG
EcoRV 457 GATATC GAT^ATC
Fnu4HI 870 GCTGC GC^[ACGT]GC
ItaI 870 GCTGC GC^[ACGT]GC
MboI 105 GATC ^GATC
MfeI 164 CAATTG C^AATTG
MunI 164 CAATTG C^AATTG
MvaI 290 CCTGG CC^[AT]GG
NdeII 105 GATC ^GATC
NspI 59 GCATGC [GA]CATG^[CT]
PleI 667 GACTC GAGTC(4/5)
RsaI 792 GTAC GT^AC
Sau3AI 105 GATC ^GATC
ScrFI 290 CCTGG CC^[ACGT]GG
SfaNI 900 GCATC GCATC(5/9)
SphI 59 GCATGC GCATG^C
SspI 447 AATATT AAT^ATT
TseI 870 GCTGC G^C[AT]GC
XbaI 661 TCTAGA T^CTAGA
XmnI 69 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcsI 46 GAATTC [GA]^AATT[CT]
636 AAATTT
ApoI 46 GAATTC [GA]^AATT[CT]
636 AAATTT
BalI 2 TGGCCA TGG^CCA
755 TGGCCA
BsaJI 617 CCTTGG C^C[ACGT][ACGT]GG
653 CCTTGG
BsiYI 683 CCCATAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
684 CCATAGGAGGG
BslI 683 CCCATAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
684 CCATAGGAGGG
BsmAI 624 GAGAC GTCTC(1/5)
665 GAGAC
BspMI 145 ACCTGC ACCTGC(4/8)
301 ACCTGC
BsrI 84 ACTGG ACTGG(1/-1)
563 CCAGT
BstF5I 138 CATCC GGATG(2/0)
374 GGATG
BstXI 5 CCACCGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
695 CCATGAAAATGG
Cfr13I 681 GGCCC G^G[ACGT]CC
692 GGGCC
EaeI 2 TGGCCA [CT]^GGCC[GA]
755 TGGCCA
EarI 548 CTCTTC CTCTTC(1/4)
724 CTCTTC
EcoT14I 617 CCTTGG C^C[AT][AT]GG
653 CCTTGG
FokI 138 CATCC GGATG(9/13)
374 GGATG
Ksp632I 548 CTCTTC CTCTTC(1/4)
724 CTCTTC
MluNI 2 TGGCCA TGG^CCA
755 TGGCCA
MscI 2 TGGCCA TGG^CCA
755 TGGCCA
MslI 54 CATCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
696 CATGAAAATG
MspA1I 128 CAGCTG C[AC]G^C[GT]G
881 CAGCTG
MwoI 814 GCCTTAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
891 GCTACTGTGGC
PvuII 128 CAGCTG CAG^CTG
881 CAGCTG
Sau96I 681 GGCCC G^G[ACGT]CC
692 GGGCC
StyI 617 CCTTGG C^C[AT][AT]GG
653 CCTTGG
TfiI 30 GATTC G^A[AT]TC
193 GATTC
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
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AluI BfaI Cac8I DdeI HaeIII
HinfI HphI MaeI MboII MnlI
MseI NlaIII Tru9I Tsp509I