Restriction map for
pFN21AE5907
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI Asp700I
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SbfI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI Tth111I Van91I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AocI 897 CCTGAGG CC^T[ACGT]AGG
AseI 858 ATTAAT AT^TAAT
AsnI 858 ATTAAT AT^TAAT
AspHI 553 GAGCTC G[AT]GC[AT]^C
AvaI 799 CTCGAG C^[CT]CG[GA]G
AvaII 698 GGTCC G^G[AT]CC
BalI 368 TGGCCA TGG^CCA
BamHI 68 GGATCC G^GATCC
BanI 436 GGTGCC G^G[CT][GA]CC
BanII 553 GAGCTC G[GA]GC[CT]^C
BbvI 4 GCAGC GCAGC(8/12)
BcnI 58 CCGGG CC^[GC]GG
BmyI 553 GAGCTC G[AGT]GC[ACT]^C
BpmI 663 CTCCAG CTGGAG(16/14)
BsaAI 364 TACGTG [CT]AC^GT[GA]
BseRI 900 GAGGAG GAGGAG(10/8)
BsgI 160 CTGCAC GTGCAG(16/14)
BsiHKAI 553 GAGCTC G[AT]GC[AT]^C
BsmBI 321 CGTCTC CGTCTC(1/5)
BsoBI 799 CTCGAG C^[CT]CG[GA]G
Bsp1286I 553 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 389 TGTACA T^GTACA
BsrGI 389 TGTACA T^GTACA
BstEII 105 GGTCACC G^GT[ACGT]ACC
BstPI 105 GGTCACC G^GT[ACGT]ACC
BstYI 68 GGATCC [GA]^GATC[CT]
Bsu36I 897 CCTGAGG CC^T[ACGT]AGG
DpnII 69 GATC ^GATC
DraIII 279 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 508 GACAACCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 368 TGGCCA [CT]^GGCC[GA]
Eco81I 897 CCTGAGG CC^T[ACGT]AGG
EcoO65I 105 GGTCACC G^GT[ACGT]ACC
EcoT14I 464 CCATGG C^C[AT][AT]GG
Fnu4HI 4 GCAGC GC^[ACGT]GC
HapII 58 CCGG C^CGG
HincII 610 GTTGAC GT[CT]^[GA]AC
HindII 610 GTTGAC GT[CT]^[GA]AC
HpaII 58 CCGG C^CGG
ItaI 4 GCAGC GC^[ACGT]GC
MboI 69 GATC ^GATC
MflI 68 GGATCC [GA]^GATC[CT]
MluNI 368 TGGCCA TGG^CCA
MscI 368 TGGCCA TGG^CCA
MspI 58 CCGG C^CGG
NciI 58 CCGGG CC^[GC]GG
NcoI 464 CCATGG C^CATGG
NdeII 69 GATC ^GATC
PaeR7I 799 CTCGAG C^TCGAG
PshBI 858 ATTAAT AT^TAAT
SacI 553 GAGCTC GAGCT^C
SapI 297 GCTCTTC GCTCTTC(1/4)
Sau3AI 69 GATC ^GATC
ScaI 712 AGTACT AGT^ACT
SspBI 389 TGTACA T^GTACA
StyI 464 CCATGG C^C[AT][AT]GG
TseI 4 GCAGC G^C[AT]GC
Tsp509I 732 AATT ^AATT
XhoI 799 CTCGAG C^TCGAG
XhoII 68 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 360 CCGC CCGC(-3/-1)
66 GCGG
AlwI 69 GATCC GGATC(4/5)
68 GGATC
BsmAI 253 GTCTC GTCTC(1/5)
322 GTCTC
BsmFI 60 GGGAC GGGAC(10/14)
841 GTCCC
BsmI 620 GAATGC GAATGC(1/-1)
668 GCATTC
BspMI 158 ACCTGC ACCTGC(4/8)
695 GCAGGT
BstNI 123 CCTGG CC^[AT]GG
411 CCAGG
DsaI 110 CCGTGG C^C[GA][CT]GG
464 CCATGG
EcoRII 123 CCTGG ^CC[AT]GG
411 CCAGG
HinfI 291 GACTC G^A[ACGT]TC
328 GAGTC
MaeII 365 ACGT A^CGT
614 ACGT
MslI 108 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
165 CACTCCCATG
MvaI 123 CCTGG CC^[AT]GG
411 CCAGG
MwoI 57 GCCGGGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
795 GCCCCTCGAGC
PleI 291 GACTC GAGTC(4/5)
328 GAGTC
SfcI 686 CTACAG C^T[GA][CT]AG
864 CTATAG
TaqI 38 TCGA T^CGA
800 TCGA
TspRI 383 CACTG CA[GC]TG[ACGT][ACGT]^
633 CACTG
TthHB8I 38 TCGA T^CGA
800 TCGA
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AfaI AluI BsaJI BsiYI BslI
BssKI BstF5I Cfr13I DdeI EarI
FokI HaeIII HphI Ksp632I MaeIII
MboII MnlI MseI NlaIII NlaIV
RsaI Sau96I ScrFI SmlI Tru9I
Tsp45I