Restriction map for
pFN21AE5928
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BanII BbeI BbrPI BbsI BcgI
BciVI BcnI BfaI BfrI BglII
BlnI BpmI BpuAI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BsrDI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraII EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MfeI MluI MroI MseI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 700 CGCG CG^CG
AflIII 203 ACATGT A^C[GA][CT]GT
AocI 617 CCTCAGG CC^T[ACGT]AGG
BamHI 677 GGATCC G^GATCC
BanI 225 GGTGCC G^G[CT][GA]CC
BbvCI 572 CCTCAGC CCTCAGC(-5/-2)
BclI 137 TGATCA T^GATCA
BglI 421 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 292 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 292 GCTCAGC GC^T[ACGT]AGC
BsaAI 97 TACGTG [CT]AC^GT[GA]
BsaBI 585 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 905 GAGAC GTCTC(1/5)
BsmFI 252 GTCCC GGGAC(10/14)
Bsp1407I 857 TGTACA T^GTACA
BspLU11I 203 ACATGT A^CATGT
BspMI 413 GCAGGT ACCTGC(4/8)
BsrBI 382 CCGCTC CCGCTC(-3/-3)
BsrFI 433 ACCGGC [GA]^CCGG[CT]
BsrGI 857 TGTACA T^GTACA
BsrI 555 ACTGG ACTGG(1/-1)
BssSI 16 CTCGTG CACGAG(-5/-1)
BstUI 700 CGCG CG^CG
BstXI 578 CCAACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 617 CCTCAGG CC^T[ACGT]AGG
Cac8I 10 GCCAGC GC[ACGT]^[ACGT]GC
CelII 292 GCTCAGC GC^T[ACGT]AGC
CfoI 699 GCGC GCG^C
Cfr10I 433 ACCGGC [GA]^CCGG[CT]
DraIII 324 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 243 GACCTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 617 CCTCAGG CC^T[ACGT]AGG
FauI 381 CCCGC CCCGC(4/6)
FbaI 137 TGATCA T^GATCA
HapII 434 CCGG C^CGG
HhaI 699 GCGC GCG^C
HinP1I 699 GCGC G^CGC
HpaII 434 CCGG C^CGG
MaeII 98 ACGT A^CGT
MamI 585 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 83 TCTTC GAAGA(8/7)
MspA1I 282 CAGCTG C[AC]G^C[GT]G
MspI 434 CCGG C^CGG
MvnI 700 CGCG CG^CG
NspI 203 ACATGT [GA]CATG^[CT]
PflMI 309 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 282 CAGCTG CAG^CTG
SfaNI 793 GATGC GCATC(5/9)
SspBI 857 TGTACA T^GTACA
StuI 39 AGGCCT AGG^CCT
Van91I 309 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 64 GACGCC G[GA]^CG[CT]C
834 GACGCC
AspHI 100 GTGCTC G[AT]GC[AT]^C
321 GAGCAC
AvaII 505 GGACC G^G[AT]CC
738 GGTCC
BbvI 284 GCTGC GCAGC(8/12)
623 GCTGC
BsaHI 64 GACGCC G[GA]^CG[CT]C
834 GACGCC
BseRI 70 CTCCTC GAGGAG(10/8)
121 CTCCTC
BsiHKAI 100 GTGCTC G[AT]GC[AT]^C
321 GAGCAC
BstYI 515 AGATCC [GA]^GATC[CT]
677 GGATCC
Cfr13I 505 GGACC G^G[ACGT]CC
738 GGTCC
DsaI 788 CCATGG C^C[GA][CT]GG
797 CCATGG
Hin1I 64 GACGCC G[GA]^CG[CT]C
834 GACGCC
HinfI 80 GAATC G^A[ACGT]TC
148 GATTC
MflI 515 AGATCC [GA]^GATC[CT]
677 GGATCC
NcoI 788 CCATGG C^CATGG
797 CCATGG
Sau96I 505 GGACC G^G[ACGT]CC
738 GGTCC
TfiI 80 GAATC G^A[AT]TC
148 GATTC
TseI 284 GCTGC G^C[AT]GC
623 GCTGC
XcmI 545 CCATCCTGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
788 CCATGGATGCCATGG
XhoII 515 AGATCC [GA]^GATC[CT]
677 GGATCC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI AlwI BalI
BmyI BsaJI BsgI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI DdeI
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HgaI HphI ItaI
MaeIII MboI MluNI MnlI MscI
MslI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI ScrFI StyI
Tsp45I TspRI