Restriction map for
pFN21AB7596
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BcgI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MscI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 796 CGCG CG^CG
AccIII 629 TCCGGA T^CCGGA
AlwNI 372 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 455 GGGCCC GGGCC^C
Asp700I 560 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 415 GAAGAC GAAGAC(2/6)
BbvCI 403 GCTGAGG CCTCAGC(-5/-2)
BglII 593 AGATCT A^GATCT
BpuAI 415 GAAGAC GAAGAC(2/6)
BsaWI 629 TCCGGA [AT]^CCGG[AT]
BseAI 629 TCCGGA T^CCGGA
BspEI 629 TCCGGA T^CCGGA
BsrI 321 CCAGT ACTGG(1/-1)
BstUI 796 CGCG CG^CG
BstYI 593 AGATCT [GA]^GATC[CT]
CfoI 797 GCGC GCG^C
EcoT14I 800 CCTTGG C^C[AT][AT]GG
HhaI 797 GCGC GCG^C
HinP1I 797 GCGC G^CGC
HincII 756 GTTGAC GT[CT]^[GA]AC
HindII 756 GTTGAC GT[CT]^[GA]AC
MaeIII 665 GTCAC ^GT[ACGT]AC
MflI 593 AGATCT [GA]^GATC[CT]
MroI 629 TCCGGA T^CCGGA
MspA1I 285 CAGCTG C[AC]G^C[GT]G
MvnI 796 CGCG CG^CG
NspI 710 GCATGC [GA]CATG^[CT]
PleI 468 GAGTC GAGTC(4/5)
PpuMI 230 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 285 CAGCTG CAG^CTG
SfcI 597 CTACAG C^T[GA][CT]AG
SphI 710 GCATGC GCATG^C
StyI 800 CCTTGG C^C[AT][AT]GG
TfiI 310 GAATC G^A[AT]TC
Tsp45I 665 GTCAC ^GT[GC]AC
XhoII 593 AGATCT [GA]^GATC[CT]
XmnI 560 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
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AspHI 10 GAGCTC G[AT]GC[AT]^C
382 GAGCTC
BbvI 491 GCAGC GCAGC(8/12)
287 GCTGC
BciVI 186 GTATCC GTATCC(6/5)
824 GTATCC
BcnI 515 CCCGG CC^[GC]GG
639 CCCGG
BfaI 106 CTAG C^TAG
817 CTAG
BsaI 207 GGTCTC GGTCTC(1/5)
338 GGTCTC
BsiHKAI 10 GAGCTC G[AT]GC[AT]^C
382 GAGCTC
EarI 222 GAAGAG CTCTTC(1/4)
391 GAAGAG
HinfI 310 GAATC G^A[ACGT]TC
468 GAGTC
HphI 26 TCACC GGTGA(8/7)
36 TCACC
Ksp632I 222 GAAGAG CTCTTC(1/4)
391 GAAGAG
MaeI 106 CTAG C^TAG
817 CTAG
MaeII 216 ACGT A^CGT
584 ACGT
MseI 86 TTAA T^TAA
556 TTAA
NciI 515 CCCGG CC^[GC]GG
639 CCCGG
NlaIII 711 CATG CATG^
837 CATG
PflMI 152 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
371 CCAGCACCTGG
SacI 10 GAGCTC GAGCT^C
382 GAGCTC
SfaNI 280 GATGC GCATC(5/9)
855 GATGC
Tru9I 86 TTAA T^TAA
556 TTAA
TseI 287 GCTGC G^C[AT]GC
491 GCAGC
TspRI 314 CACTG CA[GC]TG[ACGT][ACGT]^
832 CAGTG
Van91I 152 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
371 CCAGCACCTGG
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AvaII BanII BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HpaII ItaI
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIV Sau3AI Sau96I
ScrFI TaqI Tsp509I TthHB8I