Restriction map for
pFN21AE3054
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AluI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MaeII
MluI MseI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 572 TCCGGA T^CCGGA
AcsI 256 GAATTT [GA]^AATT[CT]
AocI 339 CCTGAGG CC^T[ACGT]AGG
ApoI 256 GAATTT [GA]^AATT[CT]
AvaII 588 GGACC G^G[AT]CC
BalI 389 TGGCCA TGG^CCA
BanII 565 GAGCCC G[GA]GC[CT]^C
BciVI 153 GTATCC GTATCC(6/5)
BsaBI 232 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 470 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 572 TCCGGA [AT]^CCGG[AT]
BseAI 572 TCCGGA T^CCGGA
BseRI 491 GAGGAG GAGGAG(10/8)
BsmBI 612 GAGACG CGTCTC(1/5)
BspEI 572 TCCGGA T^CCGGA
BsrBI 494 GAGCGG CCGCTC(-3/-3)
BssKI 157 CCTGG ^CC[ACGT]GG
BssSI 488 CACGAG CACGAG(-5/-1)
BstNI 157 CCTGG CC^[AT]GG
BstYI 160 GGATCT [GA]^GATC[CT]
Bsu36I 339 CCTGAGG CC^T[ACGT]AGG
DraII 587 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 389 TGGCCA [CT]^GGCC[GA]
Eco81I 339 CCTGAGG CC^T[ACGT]AGG
EcoO109I 587 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 157 CCTGG ^CC[AT]GG
EcoT14I 470 CCAAGG C^C[AT][AT]GG
HapII 573 CCGG C^CGG
HgaI 614 GACGC GACGC(5/10)
HinfI 606 GAATC G^A[ACGT]TC
HpaII 573 CCGG C^CGG
MamI 232 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 395 CAATTG C^AATTG
MflI 160 GGATCT [GA]^GATC[CT]
MluNI 389 TGGCCA TGG^CCA
MroI 572 TCCGGA T^CCGGA
MscI 389 TGGCCA TGG^CCA
MslI 366 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 583 CAGCGG C[AC]G^C[GT]G
MspI 573 CCGG C^CGG
MunI 395 CAATTG C^AATTG
MvaI 157 CCTGG CC^[AT]GG
NspI 142 ACATGC [GA]CATG^[CT]
PpuMI 587 GGGACCT [GA]G^G[AT]CC[CT]
ScrFI 157 CCTGG CC^[ACGT]GG
SspI 135 AATATT AAT^ATT
StyI 470 CCAAGG C^C[AT][AT]GG
TfiI 606 GAATC G^A[AT]TC
XhoII 160 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwI 160 GGATC GGATC(4/5)
237 GGATC
BbvI 432 GCTGC GCAGC(8/12)
435 GCTGC
BmyI 9 GGGCAC G[AGT]GC[ACT]^C
565 GAGCCC
BpmI 18 CTGGAG CTGGAG(16/14)
450 CTGGAG
BsmAI 612 GAGAC GTCTC(1/5)
112 GTCTC
BsmFI 519 GGGAC GGGAC(10/14)
587 GGGAC
Bsp1286I 9 GGGCAC G[AGT]GC[ACT]^C
565 GAGCCC
Cfr13I 30 GGGCC G^G[ACGT]CC
588 GGACC
DdeI 149 CTCAG C^T[ACGT]AG
340 CTGAG
DpnII 161 GATC ^GATC
238 GATC
FauI 496 GCGGG CCCGC(4/6)
585 GCGGG
HphI 202 GGTGA GGTGA(8/7)
240 TCACC
MaeIII 246 GTCAC ^GT[ACGT]AC
484 GTGAC
MboI 161 GATC ^GATC
238 GATC
MboII 76 GAAGA GAAGA(8/7)
316 GAAGA
NdeII 161 GATC ^GATC
238 GATC
NlaIII 143 CATG CATG^
366 CATG
NlaIV 124 GGAACC GG[ACGT]^[ACGT]CC
587 GGGACC
Sau3AI 161 GATC ^GATC
238 GATC
Sau96I 30 GGGCC G^G[ACGT]CC
588 GGACC
SfaNI 319 GATGC GCATC(5/9)
617 GCATC
TseI 432 GCTGC G^C[AT]GC
435 GCTGC
Tsp45I 246 GTCAC ^GT[GC]AC
484 GTGAC
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AciI BsrI BstF5I Cac8I Fnu4HI
FokI HaeIII ItaI MnlI Tsp509I
TspRI